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Genome-wide identification, phylogeny, evolution, and expression patterns of MtN3/saliva/SWEET genes and functional analysis of BcNS in Brassica rapa

文献类型： 外文期刊

作者： Miao, Liming 1 ; Lv, Yanxia 1 ; Kong, Lijun 1 ; Chen, Qizhen 1 ; Chen, Chaoquan 1 ; Li, Jia 1 ; Zeng, Fanhuan 1 ; Wang, S 1 ;

作者机构： 1.Zhejiang Univ, Inst Vegetable Sci, Lab Cell & Mol Biol, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

2.Minist Agr, Lab Hort Plant Growth & Qual Regulat, Hangzhou 310058, Zhejiang, Peoples R China

3.Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Zhejiang, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Vegetable Sci, Nanjing 210014, Jiangsu, Peoples R China

5.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

关键词： Chinese cabbage (Brassica rapa syn. Brassica campestris); MtN3/saliva/SWEETs; Gene family; BcNS; Nectary; Functional analysis

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2018 年 19 卷

页码：

收录情况： SCI

摘要： Background: Members of the MtN3/saliva/SWEET gene family are present in various organisms and are highly conserved. Their precise biochemical functions remain unclear, especially in Chinese cabbage. Based on the whole genome sequence, this study aims to identify the MtN3/saliva/SWEETs family members in Chinese cabbage and to analyze their classification, gene structure, chromosome distribution, phylogenetic relationship, expression pattern, and biological functions. Results: We identified 34 SWEET genes in Chinese cabbage and analyzed their localization on chromosomes and transmembrane regions of their corresponding proteins. Analysis of a phylogenetic tree indicated that there were at least 17 supposed ancestor genes before the separation in Brassica rapa and Arabidopsis. The expression patterns of these genes in different tissues and flower developmental stages of Chinese cabbage showed that they are mainly involved in reproductive development. The Ka/Ks ratio between paralogous SWEET gene pairs of B. rapa were far less than 1. In our previous study, At2g39060 homologous gene Bra000116 (BraSWEET9, also named BcNS, Brassica Nectary and Stamen) played an important role during flower development in Chinese cabbage. Instantaneous expression experiments in onion epidermal cells showed that the gene encoding this protein is localized to the plasma membrane. A basal nectary split is the phenotype of transgenic plants transformed with the antisense expression vector. Conclusion: This study is the first to perform a sequence analysis, structures analysis, physiological and biochemical characteristics analysis of the MtN3/saliva/SWEETs gene in Chinese cabbage and to verify the function of BcNS. A total of 34 SWEET genes were identified and they are distributed among ten chromosomes and one scaffold. The Ka/Ks ratio implies that the duplication genes suffered strong purifying selection for retention. These genes were differentially expressed in different floral organs. The phenotypes of the transgenic plants indicated that BcNs participates in the development of the floral nectary. This study provides a basis for further functional analysis of the MtN3/saliva/SWEETs gene family.

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