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Molecular cloning and expression analysis of interleukin-1 beta and interleukin-1 receptor type I genes in yellow catfish (Pelteobagrus fulvidraco): Responses to challenge of Edwardsiella ictaluri

文献类型： 外文期刊

作者： Liao, Chen-Lei 1 ; Zhang, Gui-Rong 1 ; Zhu, Dong-Mei 1 ; Ji, Wei 1 ; Shi, Ze-Chao 2 ; Jiang, Rui 1 ; Fan, Qi-Xue 1 ; Wei, 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Hubei, Peoples R China

2.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China

关键词： Bacterial challenge; cDNA cloning; Edwardsiella ictaluri; Gene expression; IL-1 beta; IL-1RI; Pelteobagrus fulvidraco

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2018 年 223 卷

页码：

收录情况： SCI

摘要： Interleukin-1 beta (IL-1 beta) is one of the pivotal early pro-inflammatory cytokines, which play important roles in regulating immune response and inducing a series of inflammatory reactions to infections. Interleukin-1 type I receptor (IL-1RI) is a receptor of the IL-1 beta that can mediate IL-1-dependent activation. In this study, partial cDNA sequences of the Pf_IL-1 beta and Pf_IL-1RI genes were cloned from yellow catfish (Pelteobagrus fulvidraco). The open reading frames (ORF) of Pf_IL-1 beta and Pf_IL-1RI genes encode putative peptides of 280 and 543 amino acids, respectively. The deduced amino acid sequences of these two genes shared highly conserved structures with those from other teleosts. Quantitative real-time PCR results showed that the pf_IL-1 beta mRNA had relatively high expression levels in trunk kidney and blood, and the Pf_IL-1RI mRNA was highly expressed in blood and had relatively high expression level in liver. Ontogenetic expression analyses indicate that the Pf_IL-1 beta and Pf_IL-1RI genes may play important roles during the embryonic developmental stages. The mRNA expression levels of Pf_IL-1 beta and Pf_IL-1RI genes were up-regulated in the trunk kidney, head kidney, blood, spleen, heart and liver after Edwardsiella ictaluri challenge. Western blot analyses showed that Pf_IL-1 beta protein was highly expressed in the spleen and head kidney, but not in the fin of adult individuals. These results suggest that the Pf_IL-1 beta and Pf_IL-1RI genes may play significant roles in the immune regulation and defense against E. ictaluri in the yellow catfish.

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