The roles of fip1 and tadD in Actinobacillus pleuropneumoniae pilus biosynthesis and pathogenicity
文献类型： 外文期刊
作者： Li, Tingting 1 ; Zhang, Qiuhong 1 ; Wang, Rong 1 ; Zhang, Sihua 1 ; Pei, Jie 1 ; Li, Yaokun 1 ; Li, Lu 1 ; Zhou, Rui 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
2.Cooperat Innovat Ctr Sustainable Pig Prod, Key Lab Prevent Vet Med Hubei Prov, Wuhan 430070, Hubei, Peoples R China
3.Minist Agr & Rural Affairs Peoples Republ China, Key Lab Dev Vet Diagnost Prod, Wuhan 430070, Hubei, Peoples R China
4.Minist Sci & Technol Peoples Republ China, Int Res Ctr Anim Dis, Wuhan 430070, Hubei, Peoples R China
5.Hubei Anim Dis Prevent & Control Ctr, Wuhan 430070, Hubei, Peoples R China
关键词： Actinobacillus pleuropneumoniae; Flp pilus; flp1; tadD; Pathogenicity
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 126 卷
页码：
收录情况： SCI
摘要： Pili have been demonstrated to contribute to the pathogenicity of many bacterial pathogens. Flp pilus encoded by the tad locus belongs to the type IVb pilus. Our previous study has revealed that the intact tad locus is essential for Flp pilus formation in Actinobacillus pleuropneumoniae, a very important porcine respiratory pathogen. To further investigate the functions of Flp pilus in A. pleuropneumoniae pathogenesis, the . flp1 and tadD single deletion mutants were constructed by homologous recombination. Both of the mutant strains lost pilus on their cell surfaces. The abilities of biofilm formation, cell adhesion, resistance to phagocytosis, survival in swine whole blood, and in vivo colonization of the two mutants were significantly reduced compared with those of the parental strain. The corresponding complemented strains recovered the phenotypes. These results demonstrated that flp1 and tadD were essential for the biosynthesis of Flp pilus and that the pilus played important roles during infection of A. pleuropneumoniae.
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