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Identification of amniotic fluid metabolomic and placental transcriptomic changes associated with abnormal development of cloned pig fetuses

文献类型: 外文期刊

作者: Ao, Zheng 1 ; Li, Zicong 1 ; Wang, Xingwang 1 ; Zhao, Chengfa 1 ; Gan, Yanmin 1 ; Wu, Xiao 1 ; Zeng, Fang 3 ; Shi, Junson 1 ;

作者机构: 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China

3.South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China

4.Guangdong Wens Foodstuff Grp Ltd, Wens Res Inst, Yunfu, Guangdong, Peoples R China

关键词: amniotic fluid; cloned pigs; metabolomics; placenta; transcriptomics

期刊名称:MOLECULAR REPRODUCTION AND DEVELOPMENT ( 影响因子:2.609; 五年影响因子:3.47 )

ISSN: 1040-452X

年卷期: 2019 年 86 卷 3 期

页码:

收录情况: SCI

摘要: Piglets cloned by somatic cell nuclear transfer (SCNT) show a high incidence of malformations and a high death rate during the perinatal period. To investigate the underlying mechanisms for abnormal development of cloned pig fetuses, we compared body weight, amniotic fluid (AF) metabolome, and placental transcriptome between SCNT- and artificial insemination (AI)-derived pig fetuses. Results showed that the body weight of SCNT pig fetuses was significantly lower than that of AI pig fetuses. The identified differential metabolites between the two groups of AF were mainly involved in bile acids and steroid hormones. The levels of all detected bile acids in SCNT AF were significantly higher than those in AI AF. The increase in the AF bile acid levels in SCNT fetuses was linked with the downregulation of placental bile acid transporter expression and the abnormal development of placental folds (PFs), both of which negatively affected the transfer of bile acids from AF across the placenta into the mother's circulation. Alteration in the AF steroid hormone levels in cloned fetuses was associated with decreased expression of enzymes responsible for steroid hormone biosynthesis in the placenta. In conclusion, cloned pig fetuses undergo abnormal intrauterine development associated with alteration of bile acid and steroid hormone levels in AF, which may be due to the poor development of PFs and the erroneous expression of bile acid transporters and enzymes responsible for steroid hormone biosynthesis in the placentas.

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