Identification of amniotic fluid metabolomic and placental transcriptomic changes associated with abnormal development of cloned pig fetuses
文献类型： 外文期刊
作者： Ao, Zheng 1 ; Li, Zicong 1 ; Wang, Xingwang 1 ; Zhao, Chengfa 1 ; Gan, Yanmin 1 ; Wu, Xiao 1 ; Zeng, Fang 3 ; Shi, Junson 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Guangdong, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China
4.Guangdong Wens Foodstuff Grp Ltd, Wens Res Inst, Yunfu, Guangdong, Peoples R China
关键词： amniotic fluid; cloned pigs; metabolomics; placenta; transcriptomics
期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）
ISSN： 1040-452X
年卷期： 2019 年 86 卷 3 期
页码：
收录情况： SCI
摘要： Piglets cloned by somatic cell nuclear transfer (SCNT) show a high incidence of malformations and a high death rate during the perinatal period. To investigate the underlying mechanisms for abnormal development of cloned pig fetuses, we compared body weight, amniotic fluid (AF) metabolome, and placental transcriptome between SCNT- and artificial insemination (AI)-derived pig fetuses. Results showed that the body weight of SCNT pig fetuses was significantly lower than that of AI pig fetuses. The identified differential metabolites between the two groups of AF were mainly involved in bile acids and steroid hormones. The levels of all detected bile acids in SCNT AF were significantly higher than those in AI AF. The increase in the AF bile acid levels in SCNT fetuses was linked with the downregulation of placental bile acid transporter expression and the abnormal development of placental folds (PFs), both of which negatively affected the transfer of bile acids from AF across the placenta into the mother's circulation. Alteration in the AF steroid hormone levels in cloned fetuses was associated with decreased expression of enzymes responsible for steroid hormone biosynthesis in the placenta. In conclusion, cloned pig fetuses undergo abnormal intrauterine development associated with alteration of bile acid and steroid hormone levels in AF, which may be due to the poor development of PFs and the erroneous expression of bile acid transporters and enzymes responsible for steroid hormone biosynthesis in the placentas.
- 相关文献
作者其他论文 更多>>
-
Analysis of differential transcriptome expression reveals that ISG15 provides support for embryo development by promoting angiogenesis in porcine mesometrium
作者：Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Hong, Linjun
关键词：Pig; Mesometrium; Transcriptome; Interferon response; ISG15
-
GPCPD1 is regulated by METTL3/IGF2BP3 dependent m6A modification to affect pig muscle fiber type conversion
作者：Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Tan, Baohua;Xu, Weihua;Zhao, Yaolu;Dai, Lihe
关键词：GPCPD1; Pig; m 6 A; Myogenic differentiation; Muscle fiber type conversion
-
Genome-Wide Association Study Reveals Genetic Mechanisms Underlying Intersex and Aproctia in Large White Pigs
作者：Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Wu, Jie;Yang, Ming;Wu, Zhenfang;Cai, Gengyuan;Wu, Zhenfang;Cai, Gengyuan
关键词：intersex; aproctia; chip data; imputed data; mixed linear models; genome-wide association studies
-
Transcriptome analysis of mRNA and miRNA in the development of LeiZhou goat muscles
作者：Fu, Junjie;Liu, Jie;Deng, Ming;Liu, Guangbin;Sun, Baoli;Guo, Yongqing;Liu, Dewu;Li, Yaokun;Liu, Jie;Zou, Xian;Fu, Junjie;Liu, Jie;Deng, Ming;Liu, Guangbin;Sun, Baoli;Guo, Yongqing;Liu, Dewu
关键词：
-
A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle
作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang
关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis
-
Multi-omic characterization of allele-specific regulatory variation in hybrid pigs
作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming
关键词：
-
Protein Dynamic Landscape during Mouse Mammary Gland Development from Virgin to Pregnant, Lactating, and Involuting Stages
作者：Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wang, Wenjing;Wang, Shunbo;Wang, Hao;Zheng, Enqin;Wu, Zhenfang;Li, Zicong;Wu, Zhenfang;Li, Zicong
关键词：development; mammary gland; mouse; DIA-based quantitativeproteomics; EGF
