Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation
文献类型： 外文期刊
作者： Wang, Ying 1 ; Gao, Ying Nv 1 ; Bai, Rui 2 ; Chen, Hong Yu 1 ; Wu, Ying Ying 1 ; Shang, Jun Jun 1 ; Bao, Da Peng 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Key Lab Appl Mycol Resources & Utilizat,Inst Edib, Minist Agr,Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci, Shanghai 201306, Peoples R China
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2019 年 9 卷
页码：
收录情况： SCI
摘要： Fungal immunoregulatory proteins (FIP) are effective small molecule proteins with broad-spectrum immunomodulatory and anti-cancer activities and can be potential agents for the development of clinical drugs and health food additives. In this study, a new member of FIP named FIP-bbo was obtained through Botryobasidium botryosum genome mining. FIP-bbo has the typical characteristics of FIP but is genetically distant from other FIPs. Recombinant FIP-bbo (rFIP-bbo) was produced in an optimized E. coli expression system, and the pure protein was isolated using a Ni-NTA column. Antineoplastic experiments suggested that FIP-bbo is similar to LZ-8 in inhibiting various cancer cells (Hela, Spac-1, and A549) at lower concentrations, but it is not as potent as LZ-8. The molecular mechanism by which FIP-bbo, FIP-fve, and LZ-8 are cytotoxic to cancer cells has been discussed based on molecular dynamics simulation. Point mutations that may improve the thermal stability of FIP-fve and FIP-bbo were predicted. These results not only present a new candidate protein for the development of anticancer adjuvants, but also provide an approach for designing FIPs with high anticancer activity.
- 相关文献
作者其他论文 更多>>
-
Effectiveness of Different Organic Solvent Additions to Water Samples for Reducing the Adsorption Effects of Organic Pesticides Using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
作者：Liu, Yucan;Xu, Xinyi;Wang, Ying;Zhang, Yan;Lu, Jianbo;Liu, Chengbin;Duan, Jinming;Sun, Hongwei
关键词：adsorption effect; UHPLC-ESI-MS/MS; organic pesticides; direct injection technique; detection signal intensity; organic solvent; addition ratio
-
OsMAPKKK5 affects brassinosteroid signal transduction via phosphorylating OsBSK1-1 and regulates rice plant architecture and yield
作者：Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Yan, Peiwen;Wang, Ying;Cui, Jinhao;Liu, Mingyu;Zhu, Yu;Ma, Fuying;Liu, Yahui;Lan, Dengyong;Dong, Shiqing;Zhang, Xinwei;He, Shicong;Hu, Jian;Yuan, Xinyu;Li, Yizhen;Yang, Jinshui;Luo, Xiaojin;Wang, Ying;Wang, Ying;Hu, Zejun;Niu, Fuan;Cao, Liming;Liu, Yang;Luo, Xiaojin
关键词：brassinosteroids; mitogen-activated protein kinase kinase kinase; crosstalk; plant architecture; rice yield
-
Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase
作者：Wang, Ying;Chen, Wenjing;Chen, Hongyu;Gong, Ming;Shao, Youran;Bao, Dapeng;Zou, Gen;Chen, Wenjing;Wang, Li
关键词：Mycoprotein; CRISPR/Cas9; Molecular design breeding; Endo; N -acetyl-beta-D-glucosaminidase; Cordyceps militaris
-
The Exploration of Cordyceps militaris Extract as a Postharvest Preservative for Flammulina filiformis
作者：Chen, Wenjing;Chen, Wenjing;Li, Yan;Shao, Youran;Chen, Hongyu;Gong, Ming;Wang, Ying;Zou, Gen;Wang, Li;Li, Nanyi
关键词：
Flammulina filiformis ;Cordyceps militaris ; bacteriostasis; antioxidation; preservation -
Multilayered regulation of autophagy-related protein kinase in Cordyceps militaris
作者：Wang, Yating;Li, Nanyi;Wang, Ying;Li, Bing;Shao, Youran;Chen, Hongyu;Gong, Ming;Zhang, Ruijing;Liu, Yu;Chen, Wenjing;Zou, Gen
关键词：Cordyceps militaris; Autophagy; Fruiting body; Pathogenicity; Secondary metabolism
-
Cross-Kingdom DNA Methylation Dynamics: Comparative Mechanisms of 5mC/6mA Regulation and Their Implications in Epigenetic Disorders
作者：Liu, Yu;Sun, Shujing;Liu, Yu;Wang, Ying;Bao, Dapeng;Chen, Hongyu;Gong, Ming;Zou, Gen
关键词：DNA methylation; 5mC; 6mA; epigenetic regulation; chromatin architecture
-
RNA-seq-based transcriptome profiling of early fruit development in Chieh-qua and analysis of related transcription factors
作者：Du, Xuan;Liu, Na;Lu, Panling;Wang, Ying;Tian, Shoubo;Lu, Bo;Zhang, Zhaohui
关键词：Transcriptome; Early fruit development; Chieh-qua; Transcription factors; MYB_superfamily
