Total Biosynthesis of Mutaxanthene Unveils a Flavoprotein Monooxygenase Catalyzing Xanthene Ring Formation
文献类型： 外文期刊
作者： Xiang, Lang 1 ; Shi, Jing 1 ; Zhu, Ao 1 ; Xu, Zi Fei 1 ; Liu, Shuang He 1 ; Wang, Yi Shuang 1 ; Guo, Zhi Kai 2 ; Jiao, Rui Hua 1 ; Tan, Ren Xiang 1 ; Ge, Hui Ming 1 ;
作者机构： 1.Nanjing Univ, Sch Life Sci, Chem & Biomed Innovat Ctr ChemBIC, Inst Funct Biomol,State Key Lab Pharmaceut Biotech, Nanjing 210023, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Peoples R China
关键词： Biosynthesis; Flavoprotein Monooxygenases; Oxidative Rearrangement; Total Biosynthesis; Xanthene
期刊名称：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION （ 影响因子：16.6； 五年影响因子：15.3 ）
ISSN： 1433-7851
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Flavoprotein monooxygenases (FPMOs) play important roles in generating structural complexity and diversity in natural products biosynthesized by type II polyketide synthases (PKSs). In this study, we used genome mining to discover novel mutaxanthene analogues and investigated the biosynthesis of these aromatic polyketides and their unusual xanthene framework. We determined the complete biosynthetic pathway of mutaxathene through in vivo gene deletion and in vitro biochemical experiments. We show that a multifunctional FPMO, MtxO4, catalyzes ring rearrangement and generates the required xanthene ring through a multistep transformation. In addition, we successfully obtained all necessary enzymes for in vitro reconstitution and completed the total biosynthesis of mutaxanthene in a stepwise manner. Our results revealed the formation of a rare xanthene ring in type II polyketide biosynthesis, and demonstrate the potential of using total biosynthesis for the discovery of natural products synthesized by type II PKSs.
- 相关文献
作者其他论文 更多>>
-
Carbazomycins I and J, two novel carbazole alkaloids isolated from Saccharopolyspora phattalungensis
作者：Wang, Wen;Zhao, Er Juan;Peng, Ting;Yan, Zhang Yuan;Jiao, Rui Hua;Guo, Zhi Kai;Guo, Zhi Kai
关键词：Microbial Natural Products; Carbazole alkaloids; Structural elucidation; Gene cluster; Biosynthesis pathway
-
Isolation and biological activity of six new polyketide and terpenoid derivatives from Neopestalotiopsis Clavispora AL01
作者：Abulaizi, Ailiman;Wang, Zi Ru;Ge, Hui Ming;Abulaizi, Ailiman;Xiong, Zi Jun;Zhang, Shi Qing;Yuan, Jing Zhe;Chen, Bi-Ting;Guo, Zhi Kai;Abulaizi, Ailiman;Xiong, Zi Jun;Zhang, Shi Qing;Yuan, Jing Zhe;Chen, Bi-Ting;Guo, Zhi Kai;Yang, Yang;Yang, Yang
关键词：Natural products; Fungus; Neopestalotiopsis clavispora; Secondary metabolites; Biological activity
-
Nagimycins A and B, Antibacterial Ansamycin-Related Macrolactams from Streptomyces sp. NA07423
作者：Guo, Zhi Kai;Abulaizi, Ailiman;Xiong, Zi Jun;Zhang, Shi Qing;Guo, Zhi Kai;Abulaizi, Ailiman;Xiong, Zi Jun;Zhang, Shi Qing;Wang, Yong Chao;Tan, Ying Zi;Abulaizi, Ailiman;Shi, Jing;Yang, Yang;Yang, La Ying;Yang, Yang;Yang, La Ying
关键词：
-
Biosynthesis of Sordarin Revealing a Diels-Alderase for the Formation of the Norbornene Skeleton
作者：Liu, Shuang He;Sun, Jia Li;Hu, Yi Ling;Zhang, Li;Zhang, Xuan;Yan, Zhang Yuan;Guo, Xing;Jiao, Rui Hua;Zhang, Bo;Tan, Ren Xiang;Ge, Hui Ming;Guo, Zhi Kai
关键词：Biosynthesis; Diels-Alderase; Glycosides; Heterologous Expression; Terpenoids
-
Resistance and phylogeny guided discovery reveals structural novelty of tetracycline antibiotics
作者：Li, Ling Yu;Hu, Yi Ling;Sun, Jia Lin;Yu, Long Bo;Shi, Jing;Wang, Zi Ru;Zhang, Bo;Guo, Wen Jie;Tan, Ren Xiang;Ge, Hui Ming;Guo, Zhi Kai
关键词：
-
Biocatalytic C14-Hydroxylation on Androstenedione Enabled Modular Synthesis of Cardiotonic Steroids
作者：Zhao, Yang;Zhang, Bo;Sun, Zi Qian;Wang, Wen;Wang, Zi Ru;Tan, Ren Xiang;Ge, Hui Ming;Zhang, Hao;Yu, Shouyun;Guo, Zhi Kai
关键词：cardiotonic steroid; P450; biocatalysis; bufotalin; digitoxigenin
-
An NADPH-Dependent Ketoreductase Catalyses the Tetracyclic to Pentacyclic Skeletal Rearrangement in Chartreusin Biosynthesis
作者：Jiao, Fang Wen;Wang, Yi Shuang;You, Xue Ting;Yang, Cheng Long;Zhang, Bo;Tan, Ren Xiang;Jiao, Rui Hua;Ge, Hui Ming;Wang, Yi Shuang;Tan, Ren Xiang;Wei, Wanqing;Chen, Yu;Liang, Yong;Guo, Zhi Kai
关键词：Baeyer-Villiger monooxidase; biosynthesis; chartreusin; ketoreductase; skeletal rearrangement
