RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs
文献类型： 外文期刊
作者： Xiao, Liyao 1 ; Qiao, Jiaxin 1 ; Huang, Yiyang 1 ; Tan, Baohua 1 ; Hong, Linjun 1 ; Li, Zicong 1 ; Cai, Gengyuan 1 ; Wu, Zhenfang 1 ; Zheng, Enqin 1 ; Wang, Shanshan 1 ; Gu, Ting 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510000, Peoples R China
2.State Key Lab Conservat & Utilizat Subtrop Agro B, Guangzhou 510000, Peoples R China
3.Guangdong Prov Lab Lingnan Modern Agr Sci & Techn, Guangzhou 510000, Peoples R China
4.Guangdong Prov Key Lab Agro Anim Genom & Mol Bree, Guangzhou 510000, Peoples R China
5.Guangdong Wens Breeding Swine Technol Co Ltd, Yunfu 527400, Peoples R China
6.Hubei Univ, Coll Life Sci, Wuhan 430000, Peoples R China
关键词： RASGRP1; H3K27me3; cell proliferation; myoblasts; skeletal muscle
期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.7； 五年影响因子：3.9 ）
ISSN： 1672-9145
年卷期： 2024 年 56 卷 3 期
页码：
收录情况： SCI
摘要： Skeletal muscle is not only the largest organ in the body that is responsible for locomotion and exercise but also crucial for maintaining the body's energy metabolism and endocrine secretion. The trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important histone modifications that participates in muscle development regulation by repressing the transcription of genes. Previous studies indicate that the RASGRP1 gene is regulated by H3K27me3 in embryonic muscle development in pigs, but its function and regulatory role in myogenesis are still unclear. In this study, we verify the crucial role of H3K27me3 in RASGRP1 regulation. The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells (PSCs). The results of qPCR, western blot analysis, EdU staining, CCK-8 assay and immunofluorescence staining show that overexpression ofRASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models, while knockdown of RASGRP1 leads to the opposite results. These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mice and pigs.
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