CLONING AND BIOINFORMATIC ANALYSIS OF A NOVEL THIOLASE II GENE (BPLTHI2) FROM BETULA PLATYPHYLLA
文献类型： 外文期刊
作者： Sun, Sheng 1 ; Kang, Xiu-Ping 1 ; Tian, Yong-Sheng 2 ; Zheng, Shao-Wen 1 ; Hao, Rui-Jie 1 ; Liu, Qun-Long 1 ; Zhang, J 1 ;
作者机构： 1.Shanxi Agr Univ, Coll Hort, Taigu, Shanxi, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China
期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）
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收录情况： SCI
摘要： A full-length cDNA sequence encoding a thiolase II of Betula platyphylla was identified from a constructed cDNA-AFLP library and obtained by the rapid amplification of cDNA ends (RACE) method. The cDNA gene, designated as BplTHI2, is 1 203 bp in length and encodes an enzyme of 400 amino acid residues with a calculated MW of 40.9 kDa. The BplTHI2 displays high similarity to the thiolase II of Camellia oleifera (90 % sequence identity) and Populus trichocarpa (89 %). It was predicted to possess two main domains, an N-terminal domain (10-268) and a C-terminal domain (278-398), which contain three predicted active sites (Cys96, His356 and Cys386). Its three-dimensional structure was predicted based on the crystal structure of human mitochondria thiolase (2ib8B). Expression analysis of the BplTHI2 gene showed that it was highly expressed in female inflorescences and not expressed in male ones. We suggest that BplTHI2 is a female inflorescence-specific gene and may be responsible for the development of female inflorescence.
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