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Oral Passive Immunization of Carp Cyprinus carpio with Anti-CyHV-3 Chicken Egg Yolk Immunoglobulin (IgY)

文献类型： 外文期刊

作者： Liu, Zhenxing 1 ; Ke, Hao 2 ; Ma, Yanping 2 ; Hao, Le 2 ; Feng, Guoqing 2 ; Ma, Jiangyao 2 ; Liang, Zhiling 2 ; Li, Yugu 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China

关键词： Cyprinid herpesvirus 3;KHV;carp;yolk immunoglobulin;IgY;oral passive immunization

期刊名称：FISH PATHOLOGY （ 影响因子：0.6； 五年影响因子：0.802 ）

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收录情况： SCI

摘要： Cyprinid herpesvirus 3 (CyHV-3), also called as koi herpesvirus, has spread all over the world since its emergence in the late 1990s. CyHV-3 causes a fatal disease, which leads to a mortality rate of more than 80% in common and ornamental (koi) carp Cyprinus carpio. The novel anti-CyHV-3 strategies are urgently needed. In this report, CyHV-3 particles were concentrated and then used for the immunization of hens in order to obtain the anti-CyHV-3 egg yolk immunoglobulin (IgY). The proportion of anti-CyHV-3 IgY to total IgY in water-soluble fraction of egg york increased from 0.014% to 23.9% after three times of immunization. The anti-CyHV-3 effects of IgY were then examined by in vivo and in vitro assays. The results suggested that the anti-CyHV-3 serum of hens and the purified anti-CyHV-3 IgY from eggs could prevent common carp brain (CCB) cells from infection. After oral passive immunization with IgY, the mortality of carp significantly decreased from 85% to 50% (P < 0.05) when the fish were challenged with CyHV-3 at a dose of 40 TCID50/fish. These results will possibly pave the way for the prevention of CyHV-3 infection.

  • 相关文献

[1]Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3. Liu, Zhenxing,Hao, Le,Ma, Yanping,Liang, Zhiling,Ma, Jiangyao,Ke, Hao.

[2]First Report of Occurrences of Two Cyprinid Herpesvirus 3 Variants, I++ II- and I++ II+Delta, in China. Liu, Zhenxing,Ke, Hao,Ma, Yanping,Hao, Le,Ma, Jiangyao,Liang, Zhiling,Chen, Zhongjian,Liu, Zhenxing,Ke, Hao,Ma, Yanping,Hao, Le,Ma, Jiangyao,Liang, Zhiling.

[3]Analysing codon usage bias of cyprinid herpesvirus 3 and adaptation of this virus to the hosts. Ma, Y. P.,Liu, Z. X.,Hao, L.,Ma, J. Y.,Liang, Z. L.,Ke, H.,Li, Y. G..

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