Characterization and high expression of recombinant Ustilago maydis xylanase in Pichia pastoris
文献类型: 外文期刊
作者: Han, Hongjuan 1 ; You, Shuang 1 ; Zhu, Bo 1 ; Fu, Xiaoyan 1 ; Sun, Baihui 1 ; Qiu, Jin 1 ; Yu, Chengye 1 ; Chen, Lei 1 ; Pen 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai
关键词: Codon optimization;Enzyme characterization;High expression;Pichia pastoris;Xylanase
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A recombinant xylanase gene (rxynUMB) from Ustilago maydis 521 was expressed in Pichia pastoris, and the enzyme was purified and characterized. Phylogenetic analysis demonstrated that rxynUMB belongs to glycosyl hydrolase family 11. The Trp84, Trp95, Glu93, and Glu189 residues are proposed to be present at the active site. The apparent molecular mass of the recombinant xylananse was approximately 24 kDa, and the optimum pH and temperature were 4.3 and 50 A degrees C, respectively. Xylanase activity was enhanced by 166 and 115 % with Fe2+ and Mn2+, respectively. The biochemical properties of this recombinant xylanase suggest that it may be a useful candidate for a variety of commercial applications.
- 相关文献
作者其他论文 更多>>
-
Synthesis of betanin by expression of the core betalain biosynthetic pathway in carrot
作者:Wang, Bo;Wang, Yahui;Deng, Yuanjie;Xiong, Aisheng;Wang, Bo;Yao, Quanhong
关键词:Daucus carota L.; Betanin; Biosynthesis; Metabolic engineering; Bioforti fication
-
HRMS analysis of pesticides in vegetables from Shanghai and risk assessment
作者:Si, Wenshuai;Wu, Nan;Yang, Haifeng;Liu, Haiyan;Jin, Xiaofen;Chen, Lei;Huang, Zhiying;Wang, Shouying;Bai, Bing;Si, Wenshuai;Si, Wenshuai;Huo, Kaixuan;Yang, Haifeng;Liu, Haiyan;Jin, Xiaofen;Chen, Lei;Huang, Zhiying;Wang, Shouying
关键词:Leafy vegetables; pesticides; dietary risk assessment; UPLC-Q/Orbitrap-HRMS
-
Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice
作者:Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong
关键词:2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae
-
Biosynthesis of melatonin from
l -tryptophan by an engineered microbial cell factory作者:Wang, Lijuan;Deng, Yongdong;Gao, Jianjie;Wang, Bo;Han, Hongjuan;Li, Zhenjun;Zhang, Wenhui;Wang, Yu;Fu, Xiaoyan;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing;Wang, Lijuan;Deng, Yongdong;Peng, Rihe;Yao, Quanhong;Tian, Yongsheng;Xu, Jing
关键词:Biosynthesis; Melatonin;
l -Tryptophan (l -Trp); Escherichia coli; Metabolic engineering -
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者:Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词:2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
-
Development of a Zeolite H-ZSM-5-Based D-mu SPE Method for the Determination of Organophosphorus Pesticides in Tea Beverages
作者:Bai, Bing;Wu, Nan;Yang, Haifeng;Liu, Haiyan;Jin, Xiaofen;Chen, Lei;Huang, Zhiying;Zhou, Changyan;Wang, Shouying;Si, Wenshuai;Yang, Haifeng;Liu, Haiyan;Jin, Xiaofen;Chen, Lei;Huang, Zhiying;Wang, Shouying;Si, Wenshuai;Si, Wenshuai
关键词:zeolite H-ZSM-5; tea beverage; organophosphorus pesticides; dispersive solid-phase extraction; HPLC-MS; MS
-
Comparative Analysis of the Quality in Ripe Fruits of Cuiguan Pear from Different Regions
作者:Zhang, Miaoqiang;Wang, Liang;Bai, Bing;Chen, Lei;Liu, Haiyan;Jin, Qiqi;Feng, Tao
关键词:Cuiguan pear; physicochemical parameters; volatile organic compounds; PCA; gas chromatography-ion mobility spectrometry (GC-IMS)