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Yeast expressed foldable quadrivalent A beta 15 elicited strong immune response against A beta without A beta-specific T cell response in wild C57BL/6 mice

文献类型： 外文期刊

作者： Tan, Lin 1 ; Wang, Hao 3 ; Tan, Xin 4 ; Zou, Juntao 1 ; Yao, Zhibin 1 ;

作者机构： 1.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Anat & Neurobiol, Guangzhou 510275, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou, Hainan, Peoples R China

3.Guangzhou Blood Ctr, Guangzhou, Guangdong, Peoples R China

4.Hainan Univ, Coll Agr, Haikou, Hainan, Peoples R China

关键词： Alzheimer disease (AD);P. pastoris;r4 x A beta 15 vaccine;T cellular immune response;C57BL/6 mice

期刊名称：HUMAN VACCINES & IMMUNOTHERAPEUTICS （ 影响因子：3.452； 五年影响因子：3.483 ）

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收录情况： SCI

摘要： Active and passive immunizations with A beta and A beta antibodies successfully reduced AD pathology and improved cognitive functions in an AD mouse model. However, human clinical trials of vaccination with synthetic A beta (AN1792), were halted due to brain inflammation, presumably induced by T cell-mediated immune response. In this study, we used Picha pastoris to produce a recombinant peptide vaccine, r4 x A beta 15 (recombinant 4 x A beta 15), four tandem repeats of A beta (1-15) interlinked by spacers. Wild-type mice were injected subcutaneously with CFA/IFA as adjuvant. r4 x A beta 15 vaccine elicited high titer anti-A beta antibodies which bound to A beta plaque in brain tissue from Tg2576 mouse. The antibody isotype was mainly IgG(1), indicating anti-inflammatory Th2 type. There was no splenocyte proliferation against A beta peptide, which indicates that the r4 x A beta 15 vaccine does not induce A beta-specific T cellular immune response. Thus, r4 x A beta 15 vaccine may be a safe and efficient vaccine for AD.

  • 相关文献

[1]用P. pastoris的pAOX1表达系统表达木糖异构酶. 易国辉,尹慧祥,张爱联. 2011

[2]Inducible expression of human angiostatin by AOXI promoter in P. pastoris using high-density cell culture. Zhang, Ai-Lian,Qu, Zhi,Yi, Guo-Hui,Su, Dong-Xiao,Tu, Fa-Zhi,Pan, Ying-Wen,Zhang, Tian-Yuan,Luo, Jin-Xian,Zhang, Tian-Yuan,Luo, Jin-Xian,Fu, Ce-Yi.

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