广东省农业科学院机构知识库

Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3

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文献类型：外文期刊

作者： Liu, Zhenxing ¹ ; Hao, Le ¹ ; Ma, Yanping ¹ ; Liang, Zhiling ¹ ; Ma, Jiangyao ¹ ; Ke, Hao ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Heath, Guangdong Open Lab Vet Publ Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou 510640, Peoples R China

关键词： cyprinid herpesvirus 3;KHV;monoclonal antibody;ORF132;ELISA;prokaryotic expression

期刊名称：FISH PATHOLOGY （影响因子：0.6；五年影响因子：0.802 ）

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收录情况： SCI

摘要： Cyprinid herpesvirus 3 (CyHV-3), also named koi herpesvirus (KHV), is a lethal pathogen of koi and common carp Cyprinus carpio. In the present study, we described a procedure to generate monoclonal antibodies (mAbs) against the envelope protein pORF132 of CyHV-3 and application of the mAb. Three recombinant plasmids targeting the expression of one complete pORF132 and two truncated pORF132 were constructed, respectively. After induction, a truncated pORF132 was successfully expressed in a soluble form and the purified recombinant protein was used in the preparation of mAbs. Two hybridoma cell clones, 4E11 and 4B11, were obtained and mAb 4E11 reacted to CyHV-3 infected cells and not to CyHV-2 (goldfish hematopoietic necrosis virus) and IcHV-1 (channel catfish virus) infected cells. An antigen-capture ELISA based on the pair of mAb 4E11 and anti-CyHV-3 IgY was primarily constructed. This antigen-capture ELISA could distinguish between the CyHV-3 infected and uninfected fish samples.

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意见箱

Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3

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意 见 箱

作者其他论文更多>>

意见箱