An autophagy gene, HoATG5, is involved in sporulation, cell wall integrity and infection of wounded barley leaves
文献类型： 外文期刊
作者： Liu, Ning 1 ; Ning, Guo-Ao 1 ; Liu, Xiao-Hong 1 ; Feng, Xiao-Xiao 1 ; Lu, Jian-Ping 2 ; Mao, Li-Juan 3 ; Su, Zhen-Zhu; 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
3.Zhejiang Univ, Anal Ctr Agrobiol & Environm Sci, Hangzhou, Zhejiang, Peoples R China
4.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
5.Zhejiang Univ, Agr Technol Extens Ctr, Hangzhou 310058, Zhejiang, Peoples R China
关键词： Harpophora oryzae;HoATG5;Autophagy;Vegetative growth;Sporulation;Infection
期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）
ISSN：
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收录情况： SCI
摘要： The endophytic fungus Harpophora oryzae is a beneficial endosymbiont isolated from wild rice. H. oryzae can not only promote rice growth and biomass accumulation but also protect rice roots from invasion by its close relative Magnaporthe oryzae. Autophagy is a highly evolutionary conserved process from lower to higher eukaryotic organisms, and is involved in the maintenance of normal cell differentiation and development. In this study, we isolated a gene (HoATG5) which encodes an essential protein required for autophagy from the beneficial endophyte fungus H. oryzae. Using targeted gene replacement, a Delta HoATG5 mutant was generated and used to investigate the biological functions of autophagy in H. oryzae. We found that the autophagic process was blocked in the HoATG5 deletion mutant. The mutant showed increased vegetative growth and sporulation, and was sensitive to nutrient starvation. The Delta HoATG5 mutant lost its ability to penetrate and infect the wounded barley leaves. These results provide new knowledge to elaborate the molecular machinery of autophagy in endophytic fungi. (C) 2016 Elsevier GmbH. All rights reserved.
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