您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Histone acetylation and reactive oxygen species are involved in the preprophase arrest induced by sodium butyrate in maize roots

文献类型： 外文期刊

作者： Zhang, Qi 1 ; Wang, Pu 1 ; Hou, Haoli 1 ; Zhang, Hao 1 ; Tan, Junjun 1 ; Huang, Yan 1 ; Li, Yingnan 1 ; Wu, Jinping 3 ; Qiu, 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China

2.Wuchang Univ Technol, Synergy Innovat Ctr Biol Peptide Antidiabet Hubei, Engn Technol Res Ctr Biol Peptide Antidiabet Hube, Dept Pharmaceut Engn,Sch Life Sci, Wuhan, Peoples R China

3.Hubei Acad Agr Sci, Ind Crops Inst, 43 Nanhu Rd, Wuhan, Hubei Province, Peoples R China

4.Hubei Acad Agr Sci, Ind Crops Inst, 43 Nanhu Rd, Wuhan, Hubei Province,

关键词： Sodium butyrate;Maize;Histone acetylation;ROS;Cell cycle

期刊名称：PROTOPLASMA （ 影响因子：3.356； 五年影响因子：3.298 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Histone acetylation plays a critical role in controlling chromatin structure, and reactive oxygen species (ROS) are involved in cell cycle progression. To study the relationship between histone acetylation and cell cycle progression in plants, sodium butyrate (NaB), a histone deacetylase (HDAC) inhibitor that can cause a significant increase in histone acetylation in both mammal and plant genomes, was applied to treat maize seedlings. The results showed that NaB had significant inhibition effects on different root zones at the tissue level and caused cell cycle arrest at preprophase in the root meristem zones. This effect was accompanied by a dramatic increase in the total level of acetylated lysine 9 on histone H3 (H3K9ac) and acetylated lysine 5 on histone H4 (H4K5ac). The exposure of maize roots in NaB led to a continuous rise of intracellular ROS concentration, accompanied by a higher electrolyte leakage ratio and malondialdehyde (MDA) relative value. The NaB-treated group displayed negative results in both TdT-mediated dUTP nick end labelling (TUNEL) and gamma-H2AX immunostaining assays. The expression of topoisomerase genes was reduced after treatment with NaB. These results suggested that NaB increased the levels of H3K9ac and H4K5ac and could cause preprophase arrest accompanied with ROS formation leading to the inhibition of DNA topoisomerase.

  • 相关文献

[1]Characterization of porcine SKIP gene in skeletal muscle development: Polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells. Xiong, Qi,Liu, Yang,Suo, Xiaojun,Zhang, Nian,Li, Xiaofeng,Yang, Qianping,Chen, Mingxin,Chai, Jin,Deng, Changyan,Jiang, Siwen,Zheng, Rong,Chai, Jin,Deng, Changyan,Jiang, Siwen,Zheng, Rong,Huang, Tao. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台