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MOLECULAR ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN BIRCH (BETULA PLATYPHYLLA) INFLORESCENCE

文献类型： 外文期刊

作者： Sun, Sheng 1 ; Kang, Xiu-Ping 1 ; Tian, Yong-Sheng 2 ; Zheng, Shao-Wen 1 ; Hao, Rui-Jie 1 ; Liu, Qun-Long 1 ; Zhang, J 1 ;

作者机构： 1.Shanxi Agr Univ, Coll Hort, Taigu, Shanxi, Peoples R China

2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China

关键词： Betula platyphylla;inflorescence;cDNA-AFLP;differential expression

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2012 年 26 卷 2 期

页码：

收录情况： SCI

摘要： Birch (Betula platyphylla) has been widely applied in furniture, paper-making, and architecture for its special properties. One of the major problems is it has a long juvenile phase which limits the possibilities for production and application in these industries. To date, the molecular mechanism and biochemical pathways of the long juvenile phase are poorly understood. In this study, cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) technique was used to analyze genes specifically expressed in the male or female inflorescence of birch. More than 7,000 TDFs (Transcript Derived Fragments) were screened by using 100 primer combinations. Of them, 168 male and 103 female inflorescence specific TDFs were isolated, subcloned and sequenced. About 54% of these TDFs were found to be similar with known genes in NCBI databases. Through GO annotation and KEGG pathway analysis, the results indicated that the male inflorescence of B. platyphylla possesses nearly two-fold more transcripts than the female inflorescence in energy production and conversion.

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