您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Protective effect of CpG-DNA against mastitis induced by Escherichia coli infection in a rat model

文献类型： 外文期刊

作者： Zhu, Yumin 1 ; Fan, Hongjie 1 ; Miao, Jinfeng 1 ; Zou, Sixiang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

2.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

关键词： Escherichia coli;mas;titis;CpG-DNA;rat;model

期刊名称：VETERINARY JOURNAL （ 影响因子：2.688； 五年影响因子：2.857 ）

ISSN： 1090-0233

年卷期： 2008 年 175 卷 3 期

页码：

收录情况： SCI

摘要： A mastitis model in rats, induced by Escherichia coli infection, was established and the protective effect of Cytosine-phosphate-Guanosine (CpG)-DNA was determined. An E coli suspension containing either 2 x 10(3) colony forming units (CFU) mL(-1)(EL group), 2 x 10(5) CFU mL(-1) (EH group), or (as controls) 100 mu l phosphate buffer saline (CON group), was inoculated into the mammary glands 72 h after parturition. The rats were euthanased 24 h post-infection. The histopathological changes in mammary tissue in the EL group were mild, whereas the structural changes in the EH group were severe and polymorphonuclear leukocytes (PMNs) had accumulated in the mammary alveoli. Interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha and N-acetyl-beta-D-glucosaminidase (NAGase) were significantly increased in the mammary tissue from the EH group but not significantly changed in the EL group. On the basis of these findings, the potential protective effect of CpG-DNA on mammary glands was tested using a 2 x 10(5) CFU mL(-1) suspension. An intramuscular injection of either CpG-DNA (200 mu g) or PBS (100 mu L) was given immediately after parturition. At 72 h post-parturn, 2 x 10(5) CFU mL(-1) E coli (100 mu L) were inoculated into the mammary glands of all rats. At pre-infection (0 h), and 8, 16, 24, 48 and 72 h after inoculation six rats were euthanased. CpG-DNA induced more rapid migration of PMNs from the blood to mammary tissue at the initial stage of infection, stimulated the secretion of IL-6 and TNF-alpha at different time points, reduced viable E coli in mammary tissues and decreased the activity of NAGase. CpG-DNA also promoted the expression of its specific receptor TLR-9 mRNA in mammary tissue. The study showed that CpG-DNA protected against E coli mastitis in this rat model. (c) 2007 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Retinoid protects rats against neutrophil-induced oxidative stress in acute experimental mastitis. Gu, Beibei,Zhu, Wei,Miao, Jinfeng,Deng, Yu'E,Zou, Sixiang,Zhu, Yumin. 2009

[2]Protective effect of retinoid against endotoxin-induced mastitis in rats. Gu, B. -B.,Miao, J. -F.,Deng, Y. -E.,Zou, S. -X.,Zhu, Y. -M.. 2009

[3]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Ziping Zhu,Na Li,Jinbin Wang. 2012

[4]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Ziping Zhu,Na Li,Jinbin Wang,Xueming Tang. 2012

[5]Study on Ecological Agricultural Model Suitable for Metropolis Characteristics--Research on the Model of Shanghai Metropolis Ecological Agriculture. LU Weiguang,ZHAO Jingyin,ZHANG Zhangeng. 2003

[6]Establishment and Analysis of the Fermentation Model of Phellinus Igniarius. Zhu, Ziping,Wang, Jinbin,Tang, Xueming,Li, Na. 2012

[7]Isolation and characterization of an oilseed rape MAP kinase BnMPK3 involved in diverse environmental stresses. Yu, SW,Zhang, LD,Zuo, KJ,Tang, DQ,Tang, KX.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台