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Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

文献类型： 外文期刊

作者： Peng, RH 1 ; Yao, QH 2 ; Xiong, AS 2 ; Cheng, ZM 2 ; Li, Y 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Agr Biotechnol Res Ctr, Shanghai, Peoples R China

2.Shanghai Acad Agr Sci, Key Lab Agr Genet & Breeding, Agr Biotechnol Res Ctr, Shanghai, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA

关键词： Agrobacterium-mediated transformation;Aspergillus phytase;Brassica napus;codon modification;endoplasmic reticulum retention signal

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2006 年 25 卷 2 期

页码：

收录情况： SCI

摘要： Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzyme can be glycosylated in the seeds of transgenic plants and retain a high stability during storage. These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems.

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