文献类型： 外文期刊
作者： Cao, MX 1 ; Huang, JQ 2 ; Yao, QH 2 ; Liu, SJ 2 ; Wang, CL 2 ; Wei, ZM 3 ;
作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol
关键词： NITS;cell-permeable Cre recombinase;marker-free;transgenic rice plants;biosafety
期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）
ISSN： 1073-6085
年卷期： 2006 年 32 卷 1 期
页码：
收录情况： SCI
摘要： The removal of selected marker genes from transgenic plants is necessary to address biosafety concerns and to carry out further experiments with transgenic organisms. In the present study, the 12-amino-acid membrane translocation sequence (MTS) from the Kaposi fibroblast growth factor (FGF)-4 was used as a carrier to deliver enzymatically active Cre proteins into living plant cells, and to produce a site-specific DNA excision in transgenic rice plants. The process, which made cells permeable to Cre recombinase-mediated DNA recombination, circumvented the need to express Cre under spatiotemporal control and was proved to be a simple and efficient system to achieve marker-free transgenic plants. The ultimate aim of the present study is to develop commercial rice cultivars free from selected marker genes to hasten public acceptance of transgenic crops.
- 相关文献
作者其他论文 更多>>
-
Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola
作者：Peng, RH;Yao, QH;Xiong, AS;Cheng, ZM;Li, Y
关键词：Agrobacterium-mediated transformation;Aspergillus phytase;Brassica napus;codon modification;endoplasmic reticulum retention signal
-
Agrobacterium-mediated multiple gene transformation in rice using a single vector
作者：Cao, MX;Huang, JQ;Wei, ZM;Yao, QH;Wan, CZ;Lu, JA
关键词：Agrobacterium tumefaciens;linked expression cassettes;multiple genes;rice;transformation
-
Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato
作者：Xiong, AS;Yao, QH;Peng, RH;Li, X;Han, PL;Fan, HQ
关键词：RNA interference;ACC oxidase;ethylene;shelf life;tomato
-
Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage
作者：Li, X;Peng, RH;Fan, HQ;Xiong, AS;Yao, QH;Cheng, ZM;Li, Y
关键词：Agrobacterium tumefaciens;cabbage;flooding tolerance;plant hemoglobin;vhb gene
-
Isolation and characterization of a cDNA encoding two novel heat-shock factor OsHSF6 and OsHSF12 in Oryza sativa L.
作者：Liu, JG;Yao, QH;Zhang, Z;Peng, RH;Xiong, AS;Xu, F;Zhu, H
关键词：heat-shock factor;OsHSF6;OsHSF12;rice;yeast one-hybrid
-
A new rice zinc-finger protein binds to the O2S box of the alpha-amylase gene promoter
作者：Peng, RH;Yao, QH;Xiong, AS;Fan, HQ;Li, X;Peng, YL;Cheng, ZM;Li, Y
关键词：rice zinc-induced protein;O2S box;yeast one-hybrid system
-
A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
作者：Xiong, AS;Yao, QH;Peng, RH;Li, X;Fan, HQ;Cheng, ZM;Li, Y
关键词：
