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Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage

文献类型： 外文期刊

作者： Li, X 1 ; Peng, RH 2 ; Fan, HQ 2 ; Xiong, AS 2 ; Yao, QH 3 ; Cheng, ZM; Li, Y;

作者机构： 1.Shanghai Acad Agr Sci, Agribiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Agribiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06268 USA

3.Shanghai Acad Agr Sci, Agribiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA; Univ Connecticut, Dept Plant

关键词： Agrobacterium tumefaciens;cabbage;flooding tolerance;plant hemoglobin;vhb gene

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2005 年 23 卷 10-11 期

页码：

收录情况： SCI

摘要： Agrobacterium tumefaciens was used to deliver the vhb gene into cabbage (Brassica oleracea var. Cabitata) cv. Xiaguangs parent line, 103. Using hypocotyls and cotyledon petioles as explants for infection, we obtained a transformation efficiency of 3-5% based on the number of transgenic shoots produced from the number of explants used for infection. Molecular analysis indicated that the vhb gene was stably integrated into the cabbage genome and that the vhb gene was expressed at the RNA level. Characterization of the vhb over-expressing transgenic plants revealed that transgenic seeds germinated faster than the wildtype controls. More importantly, the transgenic plants showed tolerance to a prolonged submergence treatment, suggesting that the vhb gene may provide an excellent tool for creation of submergence/flooding-tolerant cultivars of agriculturally important crops.

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