文献类型： 外文期刊
作者： Peng, RH 1 ; Yao, QH 2 ; Xiong, AS 2 ; Fan, HQ 2 ; Li, X 2 ; Peng, YL 2 ; Cheng, ZM 2 ; Li, Y 2 ;
作者机构： 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China; Chinese Agr Univ, Dept Plant Pathol, Beijing, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA
关键词： rice zinc-induced protein;O2S box;yeast one-hybrid system
期刊名称：EUROPEAN JOURNAL OF BIOCHEMISTRY （ 影响因子：3.579； ）
ISSN： 0014-2956
年卷期： 2004 年 271 卷 14 期
页码：
收录情况： SCI
摘要： A putative transcription factor, named RAMY, that binds to the 20-bp O2S sequences of the regulatory region of the Amy2 gene promoter has been identified using the yeast one-hybrid system from a rice library. The full length RAMY cDNA clone encodes a 218-amino acid protein and is homologous to the late embryogenesis-abundant protein (LEA5). In vitro mutagenesis and electrophoretic mobility shift assays confirmed that RAMY can bind with O2S specifically through an unusual zinc finger with a CXCX4CX2H consensus sequence. Low levels of RAMY mRNAs were detected in rice leaves and roots by Northern blot hybridization. The plant hormone gibberellin (GA) induces expression of both RAMY and Amy2 genes, as performed by Northern blot hybridization, but the increase in RAMY mRNA level occurs prior to that of the Amy2 mRNA level in the GA-treated aleurone tissues. These data suggest that RAMY may act as a trans-acting protein and is probably involved in the GA-induced expression of the rice alpha-amylase gene.
- 相关文献
作者其他论文 更多>>
-
Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase
作者：Cao, MX;Huang, JQ;Yao, QH;Liu, SJ;Wang, CL;Wei, ZM
关键词：NITS;cell-permeable Cre recombinase;marker-free;transgenic rice plants;biosafety
-
Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola
作者：Peng, RH;Yao, QH;Xiong, AS;Cheng, ZM;Li, Y
关键词：Agrobacterium-mediated transformation;Aspergillus phytase;Brassica napus;codon modification;endoplasmic reticulum retention signal
-
Agrobacterium-mediated multiple gene transformation in rice using a single vector
作者：Cao, MX;Huang, JQ;Wei, ZM;Yao, QH;Wan, CZ;Lu, JA
关键词：Agrobacterium tumefaciens;linked expression cassettes;multiple genes;rice;transformation
-
Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato
作者：Xiong, AS;Yao, QH;Peng, RH;Li, X;Han, PL;Fan, HQ
关键词：RNA interference;ACC oxidase;ethylene;shelf life;tomato
-
Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage
作者：Li, X;Peng, RH;Fan, HQ;Xiong, AS;Yao, QH;Cheng, ZM;Li, Y
关键词：Agrobacterium tumefaciens;cabbage;flooding tolerance;plant hemoglobin;vhb gene
-
Isolation and characterization of a cDNA encoding two novel heat-shock factor OsHSF6 and OsHSF12 in Oryza sativa L.
作者：Liu, JG;Yao, QH;Zhang, Z;Peng, RH;Xiong, AS;Xu, F;Zhu, H
关键词：heat-shock factor;OsHSF6;OsHSF12;rice;yeast one-hybrid
-
A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
作者：Xiong, AS;Yao, QH;Peng, RH;Li, X;Fan, HQ;Cheng, ZM;Li, Y
关键词：
