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Agrobacterium-mediated multiple gene transformation in rice using a single vector

文献类型： 外文期刊

作者： Cao, MX 1 ; Huang, JQ 2 ; Wei, ZM 2 ; Yao, QH 3 ; Wan, CZ; Lu, JA;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China

3.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201

关键词： Agrobacterium tumefaciens;linked expression cassettes;multiple genes;rice;transformation

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2005 年 47 卷 2 期

页码：

收录情况： SCI

摘要： The homodimeric hemoglobin gene (VHb), the trans-zeatin synthetase gene (tzs), the modified 5-enolpyruvylshikimate-3-phosphate synthase gene (EPSPS), a selectable marker gene (hpt), and a reporter gene (gus), as linked expression cassettes, were stacked into the T-DNA region of a binary vector and introduced simultaneously into immature embryos of the rice (Oryza sativa L.) varieties Xiushui-11, Qiufeng, Youfeng, and Hanfeng by Agrobacterium tumefaciens. A total of 1 153 transgenic lines was obtained through selection for hygromycin B resistance. Approximately 90.2% of the transgenic lines harbored all the transgenes. Integration of multiple transgenes occurred at one to three genetic loci. Expression analysis revealed that the transgenes were coexpressed and inherited in a simple Mendelian fashion in transgenic plants and the frequency of coexpression was approximately 85%. On the basis of the cointegration and coexpression of the transgenes, most transgenic families were considered to be useful in a breeding program.

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