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Chloroplast genomes of two Pueraria DC. species: sequencing, comparative analysis and molecular marker development

文献类型： 外文期刊

作者： Li, Jishuang 1 ; Yang, Meng 1 ; Li, Yanni 1 ; Jiang, Mei 1 ; Liu, Chang 1 ; He, Meijun 2 ; Wu, Bin 1 ;

作者机构： 1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, 151 Malianwa North Rd, Beijing 100193, Peoples R China

2.Hubei Acad Agr Sci, Inst Chinese Med Mat, 253 Xueyuan Rd, Enshi 445000, Hubei, Peoples R China

关键词： chloroplast genome; comparative analysis; molecular marker; Pueraria lobata (Willd; ) Ohwi; Pueraria thomsonii Benth

期刊名称：FEBS OPEN BIO （ 影响因子：2.792； 五年影响因子：2.676 ）

ISSN： 2211-5463

年卷期： 2022 年 12 卷 2 期

页码：

收录情况： SCI

摘要： Puerariae lobatae radix (Ge-Gen in Chinese) and Puerariae thomsonii radix (Fen-Ge) are widely used as medicine and health products, particularly in Chinese medicine. Puerarin and daidzein are the primary bioactive compounds in Puerariae radix. These isoflavones have been used to treat cardiovascular and cerebrovascular diseases, hypertension, diabetes, and osteoporosis. The content of puerarin in Ge-Gen is about six times higher than that in Fen-Ge, so its use has a higher pharmacological effect. It is therefore of great importance to effectively distinguish between these two species. However, because their basal plants, P. lobata (Willd.) Ohwi and P. thomsonii Benth., possess an extremely similar appearance, and detecting the level of chemical constituents is just a rough distinction, it is necessary to develop more efficient identification approaches. Here the complete chloroplast genomes of P. lobata and P. thomsonii were deciphered, including sequencing, assembly, comparative analysis, and molecular marker development. The results showed that they are 153,393 and 153,442 bp in length, respectively; both contain 124 annotated genes, including eight encoding rRNA, 29 encoding tRNA, and 87 encoding proteins. Phylogenetic analysis showed that they form a clade, indicating that they originate from the same ancestor. After obtaining 10 intergenic/intronic regions with a genetic distance greater than 0.5 cm, primers were designed to amplify regions of high variability in P. lobata and P. thomsonii. Finally, a 60-bp differential base fragment, located in the intron of rpl16, was developed as a molecular marker to efficiently distinguish between these two species.

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