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UhpA in Edwardsiella piscicida decreases the pathogenicity and the capability of inducing cytokine response in zebrafish

文献类型： 外文期刊

作者： Liu, Jinyu 1 ; Sun, Yongcan 1 ; Huang, Jinjing 1 ; Ding, Lei 1 ; Ye, Tingqi 1 ; Wang, Xuepeng 1 ;

作者机构： 1.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, Shandong Prov Engn Technol Res Ctr Anim Dis Contr, Tai An 271018, Shandong, Peoples R China

3.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Mariculture, Qingdao 266104, Peoples R China

4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol, Qingdao 272000, Peoples R China

关键词： Edwardsiella piscicida; Zebrafish; UhpA; Pathogenicity; Cytokines

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）

ISSN： 2352-5134

年卷期： 2020 年 17 卷

页码：

收录情况： SCI

摘要： Edwardsiella piscicida (E. piscicida) is one of the main pathogens of fish, which could invade the host through the gut. Glucose 6-phosphate (G1u6P) within the gut provides environmental stresses that affect the pathogenicity of bacteria. Here we focus on a novel two-component chemical signal transduction system, named UhpB/UhpA in E. piscicida, including the key component hexose phosphate transport system regulatory protein (UhpA) coded by the uhpA gene. This study will elucidate the functions of the uhpA gene in the pathogenicity of E. piscicida and the immune response in zebrafish when infected with E. piscicida. The results showed that the wild type strain of E. piscicida EIB202 demonstrated significant increases in growth compared to the mutant strain AuhpA with or without 20 mM G1u6P (P < 0.05). The LD50 of E. piscicida AuhpA strain (2 x 10(4) CFU/fish) for zebrafish was significantly lower than the wild-type strain (1 x 10(5) CFU/fish), which suggested the uhpA gene deletion could increase its virulence. Besides, fish infected with AuhpA showed significantly higher (p < 0.05) gene expression of IL-1 beta and TGF-beta in spleen than fish infected with EIB202 at 6 h, 12 h, 24 h, and 48 h after infection, TNF-alpha in spleen of fish infected with AuhpA showed significantly higher (p < 0.05) than fish infected with EIB202 at 24 h, 48 h, and 72 h after infection, and IFN-gamma in spleen of fish infected with AuhpA showed significantly higher (p < 0.05) than fish infected with EIB202 at 12 h, 24 h and 72 h after infection. In summary, these results suggest that the uhpA gene deletion in E. piscicida affects G1u6P usage; most important, which could enhance the pathogenicity of E. piscicida in zebrafish, particularly increase the induction of the gene expression of IL-15, TNF alpha, IFN-gamma and TGF-beta in the spleens of zebrafish after infection.

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