Coumarin-Based Reversible Fluorescent Probe for Selective Detection of Cu(2+)in Living Cells
文献类型： 外文期刊
作者： Ahmed, Nadeem 1 ; Zareen, Wajeeha 1 ; Zhang, Di 3 ; Yang, Xiaopeng 1 ; Ye, Yong 1 ;
作者机构： 1.Zhengzhou Univ, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China
2.Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China
3.Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Peoples R China
关键词： Fluorescent probe; Cu2+; H2S; MCF-7 cells; Fluorescence imaging
期刊名称：JOURNAL OF FLUORESCENCE （ 影响因子：2.217； 五年影响因子：1.906 ）
ISSN： 1053-0509
年卷期： 2020 年 30 卷 5 期
页码：
收录情况： SCI
摘要： Copper ion plays an important role in many biological processes in human body. H2S is considered as the third gasses transmitter after carbon monoxide and nitric oxide. Here a novel ICT-based fluorescent ON-OFF-ON probe for Cu(2+)and H2S detection was developed. Selectivity and sensitivity of probe was confirmed in aqueous Tris-HCl buffer (10 mM, pH 7.4, containing 90% acetonitrile). Probe DF-CU shows high selectivity over other analytes. The degree of fluorescence quenching is linearly associated with the concentration of Cu2+(R-2 = 0.9919). The limit of detection (LOD, calculated according to the 3 sigma/slope) for Cu(2+)was 6.4 mu M. Probe can work in almost all pH. The probe shows a very fast response to Cu2+(within 10 s). Its response to copper ion could be reversed by H2S. The complex of probe with Cu(2+)could be used for H2S detection. Furthermore, this ON-OFF-ON fluorescent probe successfully applied in the living cells for the detection of Cu(2+)and H2S.
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