文献类型： 外文期刊
作者： Tan, Baohua 1 ; Hong, Linjun 1 ; Qiao, Jiaxin 1 ; Zhou, Jian 1 ; Xing, Pingping 1 ; Yan, Guanhao 1 ; Zheng, Enqin 1 ; Cai 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China
2.Wens Foodstuff Grp Co Ltd, Yunfu, Peoples R China
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2020 年 2020 卷
页码：
收录情况： SCI
摘要： The proper methylation status of histones is essential for appropriate cell lineage and organogenesis. EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. The pig is an increasing important animal model for molecular study and pharmaceutical research. However, the transcript variant and temporal expression pattern ofEZH2in the middle and late porcine fetus are still unknown. Here, we identified the coding sequence of theEZH2gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). Our results showed that the coding sequence ofEZH2was 2241 bp, encoding 746 amino acids. There were 9 amino acid insertions and an amino acid substitution in this transcript compared with the validated reference sequence in NCBI.EZH2was ubiquitously expressed in the fetal tissues of two time points with different expression levels. These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development.
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