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Cascade DNA Circuits Mediated CRISPR-Cas12a Fluorescent Aptasensor based on Multifunctional Fe3O4@hollow-TiO2@MoS2 Nanochains for Tetracycline Determination

文献类型： 外文期刊

作者： Lv, Yan 1 ; Sun, Yuhan 1 ; Zhou, You 3 ; Khan, Imran Mahmood 1 ; Niazi, Sobia 1 ; Yue, Lin 1 ; Zhang, Yin 4 ; Wang, Zhouping 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China

4.Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China

5.Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China

6.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Peoples R China

关键词： cascaded dynamic DNA network circuits; CRISPR-Cas12a; fluorescence aptasensors; multifunctional Fe3O4@hollow-TiO2@MoS2 nanochains; photocatalytic degradation

期刊名称：SMALL （ 影响因子：13.3； 五年影响因子：13.2 ）

ISSN： 1613-6810

年卷期： 2023 年 19 卷 16 期

页码：

收录情况： SCI

摘要： Herein, for the first time, the CRISPR-Cas12a system is combined with aptamer, cascaded dynamic DNA network circuits, and Fe3O4@hollow-TiO2@MoS2 nanochains (Fe3O4@h-TiO2@MoS2 NCs) to construct an efficient sensing platform for tetracycline (TC) analysis. In this strategy, specific recognition of the target is transduced and amplified into H1-H2 duplexes containing the specific sequence of Cas12a-crRNA through an aptamer recognition module and the dual amplification dynamic DNA network. Subsequently, the obtained activated Cas12a protein non-specifically cleaves the adjacent reporter gene ssDNA-FAM to dissociate the FAM molecule from the quencher Fe3O4@h-TiO2@MoS2 NCs, resulting in the recovery of the fluorescence signal and further signal amplification. Particularly, the synthesized multifunctional Fe3O4@h-TiO2@MoS2 NCs composites also exhibit superb magnetic separability and photocatalytic degradation ability. Under optimal conditions, the aptasensor displays a distinct linear relationship with the logarithm of TC concentration, and the limit of detection is as low as 0.384 pg mL(-1). Furthermore, the results of spiked recovery confirm the viability of the proposed aptasensor for TC quantification in real samples. This study extends the application of the CRISPR-Cas12a system in the field of analytical sensing and contributes new insights into the exploration of reliable tools for monitoring and treating hazards in food and environment.

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