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Mechanism of mitigating effect of wheat germ peptides on lead-induced oxidative damage in PC12 cells

文献类型： 外文期刊

作者： Li, Ning 1 ; Wen, Liuding 1 ; Wang, Fangyu 2 ; Wang, Tianlin 1 ; Li, Tiange 1 ; Qiao, Mingwu 1 ; Song, Lianjun 1 ; Bukyei, Erkigul 3 ; Huang, Xianqing 1 ;

作者机构： 1.Henan Agr Univ, Henan Engn Technol Res Ctr Food Proc & Circulat Sa, Coll Food Sci & Technol, 63 Agr Rd, Zhengzhou 450000, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, 116 Huayuan Rd, Zhengzhou 450002, Peoples R China

3.Mongolian State Univ Life Sci, Sch Engn & Technol, Dept Food Engn & Hydromech, Zaisan 53, Ulaanbaatar 17024, Mongolia

关键词： Lead; Wheat germ peptides; Oxidative stress; PC12 cells; Neurotoxicity

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 246 卷

页码：

收录情况： SCI

摘要： It is well known that lead-induced neurotoxicity is closely related to oxidative stress. According to previous reports, wheat germ peptides (WGPs) isolated from wheat germ have been shown to have potent antioxidant capacity. This study hypothesized that WGPs could protect PC12 cells from lead-induced oxidative stress. Here, the protecting-efficacies of WGPs were investigated in PC12 cells that were pretreated with WGPs (200 mu M, 4 h) and exposed to lead (10 mu M, 24 h). The antioxidant capacity was assessed by cell viability, ROS, MDA, SOD, CAT, GR, GPx, GSH, and GSSG. The experimental results showed that WGP3, WGP8, and WGP9 could reverse the reduction of cell viability caused by lead exposure. Lead exposure causes oxidative stress by increasing the levels of ROS and MDA. Moreover, the decrease in the levels of SOD, CAT, GPx, GR, and GSH/GSSG could be observed. However, WGP3, WGP8, and WGP9 can protect PC12 cells against lead-induced oxidative stress by reversing these phenomena. The protein expression of TXNIP, Keap1, and Nrf2 was characterized by western blotting, and the results illustrated that lead exposure up-regulated the expression of TXNIP and Keapl and down-regulated the expression of Nrf2, and WGP3, WGP8, and WGP9 could improve the antioxidant capacity of PC12 cells by reversing this phenomenon. Therefore, the present study demonstrated that WGP3, WGP8, and WGP9 may protect against lead-induced oxidative stress in PC12 cells by regulating the TXNIP/Keap1/Nrf2 pathway.

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