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Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization

文献类型： 外文期刊

作者： Gou, Hongchao 1 ; Li, Juan 1 ; Cai, Rujian 1 ; Song, Shuai 1 ; Li, Miao 1 ; Yang, Dongxia 1 ; Jiang, Zhiyong 1 ; Li, Yan; 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Guangdong, Peoples R China; Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Guangdong, Peoples R China; Guangdong Open Lab Vet Publ Hlth, Guangzhou, Guangdong, Peoples R China

关键词： Haemophilus parasuis;Cross-priming amplification;Vertical flow visualization;Detection

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN： 0167-7012

年卷期： 2018 年 144 卷

页码：

收录情况： SCI

摘要： Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-priming amplification (CPA) with a vertical flow (VF) visualization strip was established to detect H. parasuis. The reaction can specifically identify 15 serovar reference strains and 57 clinically isolated strains of H. parasuis, with a detection limit of 14 CFU. The performance of the CPA-VF assay was evaluated and compared with that of species-specific PCR by testing 62 clinical culture-positive specimens of H. parasuis. The entire process, from specimen processing to analysis of the results, can be completed in 2 h without a complicated apparatus. The convenience and speed of the CPA-VF assay in this study make it a suitable choice for epidemiological investigation and point-of-care testing (POCT) for H. parasuis infection.

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[1]Development and application of a loop-mediated isothermal amplification method for rapid detection of Haemophilus parasuis. Zhang, Jian-Min,Xu, Cheng-Gang,Guo, Li-Li,Zhang, Bin,Li, Jing-Yi,Chen, Ji-Dang,Fan, Hui-Ying,Liao, Ming,Shen, Hai-Yan. 2011

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[3]Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5. Li, Miao,Li, Guoqing,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling,Li, Miao,Song, Shuai,Yang, Dongxia,Li, Chunling.

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[5]Species-specific detection of Dickeya sp (Pectobacterium chrysanthemi) in infected banana tissues, soil and water. Li, Peiqian,Lin, Birun,Shen, Huifang,Pu, Xiaoming. 2011

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