Effects of Selenylation Modification on Antioxidative Activities of Schisandra chinensis Polysaccharide
文献类型： 外文期刊
作者： Yue, Chanjuan 1 ; Chen, Jin 2 ; Hou, Ranran 1 ; Liu, Jie 1 ; Li, Xiuping 1 ; Gao, Zhenzhen 1 ; Liu, Cui 1 ; Wang, Deyun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Jiangsu, Peoples R Chi
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 7 期
页码：
收录情况： SCI
摘要： The selenylation modification of Schisandra chinensis polysaccharide (SCP) was conducted by the HNO3-Na2SeO3 method respectively under nine conditions according to L-9(3(4)) orthogonal design. Nine selenizing SCPs, sSCP(1)-sSCP(9), were obtained, and their antioxidant activities were compared. In vitro test, the free radical-scavenging rates of nine sSCPs were determined for DPPH center dot, center dot OH and ABTS(+). sSCP(1) presented the most significant effect, and could inhibit the nonenzymatic protein glycation. In vivo test, 14-day-old chickens were injected respectively with sSCP(1) and SCP, the serum contents of CAT, SOD and MDA were determined. The result showed that as compared with the SCP group, the SOD and CAT activities were significantly or numerically raised and MDA content was significantly or numerically lowered in the sSCP(1) group. These results indicate that selenylation modification can significantly enhance the antioxidant and antiglycative activity of SCP in vitro or in vivo. sSCP(1) possesses the best efficacy and its modification conditions can be as optimal modification conditions that were 200 mg of Na2SeO3 for 500 mg of SCP, reaction temperature of 50 degrees C and reaction time of 6 h.
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