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Comparison of three ELISA methods for the detection of apple chlorotic leaf spot virus and apple stem grooving virus

文献类型： 外文期刊

作者： Wu, YQ 1 ; Zhang, DM 1 ; Chen, SY 1 ; Wang, XF 1 ; Wang, WH 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, Peoples R China

关键词： tissue culture;heat therapy;apple (Malus domestica Borkh.)

期刊名称：17TH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES, VOLS 1 AND 2

ISSN： 0567-7572

年卷期： 1998 年 472 期

页码：

收录情况： SCI

摘要： Apple chlorotic leafspot virus (ACLSV) and apple stem grooving virus (ASGV) are two important viruses of apple and other fruit crops. They infect many commercial apple cultivars with infection rate of up to 80-100% and cause yield loss of up to 40% in P. R. China. Protein A sandwich enzyme-linked immunosorbent assay (PAS-ELISA) has been used as a routine method for the detection of ACLSV and ASGV and for screening of virus-free materials generated from virus elimination programs. However. this method involves more steps and thus time consuming. The standard and modified version of the double antibody sandwich ELISA (DAS-ELISA) were compared with PAS-ELISA for their effectiveness in detecting ACLSV and ASGV from tissue culture of commercially important cultivars. Results indicated that the standard DAS-ELISA was successful to detect ASGV but failed to detect ACLSV. The modified DAS-ELISA showed similar efficacy to PAS-ELISA in detecting both viruses.

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