文献类型： 外文期刊
作者： Liu, Ying 1 ; Xie, Qing-Yi 2 ; Shi, Wei 3 ; Li, Lei 4 ; An, Jun-Ying 1 ; Zhao, Ya-Mei 1 ; Hong, Kui 4 ;
作者机构： 1.Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Peoples R China
3.Guangdong Huankai Microbial Sci & Tech Co Ltd, Guangzhou 510663, Guangdong, Peoples R China
4.Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China
5.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China
期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）
ISSN： 1466-5026
年卷期： 2014 年 64 卷
页码：
收录情况： SCI
摘要： A Gram-stain positive strain, M1(T), was isolated from the sediment of Maar Lake in Zhanjiang, Guangdong Province, China. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid, and mycolic acids were not detected. The polar lipid profile of strain M1(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. The predominant quinone was MK-7, with MK-6 as a minor component. The major fatty acids were anteiso-C-15:0 and anteiso-C-17:0, with iso-C-18:0 as a minor component. The DNA G+C content of the genomic DNA was 71.0 mol%. 16S rRNA gene sequence analysis showed that strain M1(T) belongs to the family Dermabacteraceae, sharing highest sequence similarity with Brachybacterium nesterenkovii JCM 11648(T) (98.1%). Furthermore, a combination of DNA DNA relatedness and physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain M1(T) represents a novel species of the genus Brachybacterium, for which the name Brachybacterium huguangmaarense sp. nov. is proposed. The type strain is M1(T) (=CCTCC AB 2012866(T)=DSM 26370(T)).
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