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Embryo and anther regulation of the mabinlin II sweet protein gene in Capparis masaikai L,vl

文献类型： 外文期刊

作者： Hu, Xin-Wen 2 ; Liu, Si-Xin 2 ; Guo, Jian-Chun 1 ; Li, Ji-Tao 1 ; Duan, Rui-Jun 1 ; Fu, Shao-Ping 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crops Biotechnol, Minist Agr, Haikou 571101, Peoples R China

2.Hainan Univ, Haikou 571104, Peoples R China

关键词： Capparis masaikai Levl;Mabinlin II;Embryo-specific;Anther-specific regulation;ACGT motif;RY motif

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN： 1438-793X

年卷期： 2009 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Mabinlin II is one of the major sweet proteins stored in the seeds of Capparis masaikai L,vl. Its promoter region (779 bp) located 5' upstream of the mabinlin II gene has been isolated and named as MBL-779 (GenBank accession number, EU014073). This promoter contains two typical TATA box regions and a series of motifs related to seed-specific promoters, such as ACGT motifs, RY motif, napin motif, and G box. The MBL-779 promoter drove GUS gene to transiently express in the embryos of bean, maize, and rice seeds or to constantly express in the embryos and anthers of the transgenic Arabidopsis. The MBL-779 promoter regulated gene expression from approximately the 12th day and peaked on approximately the 16th day after flowering in Arabidopsis. The -300-bp promoter region is a minimal sequence required to functionally regulate gene expression. The CAATs at -325 to -322 bp and -419 to -416 bp and the region at -485 to -770 bp play a role in the quantitative regulation of gene expression. The RY motif, CATGAC, at -117 to -112 bp and the ACGT within the G box (CACGTG) at -126 to -123 bp positively regulate gene expression.

  • 相关文献

[1]Recombinant expressions of sweet plant protein mabinlin II in Escherichia coli and food-grade Lactococcus lactis. Gu, Wenliang,Xia, Qiyu,Fu, Shaoping,Guo, Jianchun,Yao, Jing,Hu, Xinwen.

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