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Marker-assisted selection for pyramiding the waxy and opaque-16 genes in maize using cross and backcross schemes

文献类型： 外文期刊

作者： Yang, Liuqi 1 ; Wang, Wei 1 ; Yang, Wenpeng 1 ; Wang, Mingchun 1 ;

作者机构： 1.Guizhou Acad Agr Sci, Guizhou Inst Upland Food Crops, Guiyang 550006, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Guizhou, Peoples R China

关键词： Waxy;Opaque-16;MAS;Lysine;Amylopectin;Maize

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2013 年 31 卷 4 期

页码：

收录情况： SCI

摘要： The waxy (wx) gene in maize is associated with higher content of amylopectin in the endosperm and better flavor. The opaque-16 (o16) gene is associated with higher lysine content in the endosperm and better nutritional value. To pyramid the wx and o16 genes, cross and backcross populations were constructed using the o16 line QCL3024 and the two waxy lines, QCL5019 and QCL5008, as parents. The linkage marker umc1141 for the o16 gene and the internal marker phi027 for the wx gene were used to select the target genes. Simple sequence repeat markers covering the whole genome were used for background selection in individual progenies of the backcross population. The grain lysine content was determined using the Acid Orange-12 Dye Binding Lysine method. Qualitative and quantitative analyses of the grain content of amylopectin were performed using the I-2-KI procedure and double-wavelength spectrophotometry, respectively. Four lines of the double recessive genotype wxwxo16o16 were obtained from the F4 generation of the cross population and three lines of the same genotype were obtained from the BC2F4 generation of the backcross population. The lysine content of the pyramid lines was 16-27 and 18-28 % higher than the waxy parents QCL5019 and QCL5008, respectively. The pyramid lines had 61-63 % more amylopectin than the high-lysine parent QCL3024. The three pyramid lines from the backcross population had similar genetic background to the waxy parent QCL5008. Our results are of significance for the improvement of maize quality.

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[2]Molecular genetic mapping of a high-lysine mutant gene (opaque-16) and the double recessive effect with opaque-2 in maize. Yang, WP,Zheng, YL,Zheng, WT,Feng, R. 2005

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