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Roles of MEF2A and MyoG in the transcriptional regulation of bovine LATS2 gene

文献类型： 外文期刊

作者： Zhang, Jiupan 1 ; Raza, Sayed Haidar Abbas 3 ; Wei, Dawei 2 ; Yaping, Song 2 ; Chao, Jiang 2 ; Jin, Wang 1 ; Almohaimeed, Hailah M. 4 ; Batarfi, Munirah A. 5 ; Assiri, Rasha 6 ; Aggad, Waheeb S. 7 ; Ghalib, Samirah H. 8 ; Ageeli, Abeer A. 8 ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750021, Peoples R China

2.Ningxia Univ, Sch Agr, 489 Helanshan West Rd, Yinchuan 750021, Peoples R China

3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

4.Princess Nourah Bint Abdulrahman Univ, Coll Med, Dept Basic Sci, POB 84428, Riyadh 84428, Saudi Arabia

5.King Saud Bin Abdulaziz Univ Hlth Sci, Coll Med, Dept Anat, Basic Med Sci, Riyadh, Saudi Arabia

6.Princess Nourah Bint Abdulrahman Univ, Coll Med, Dept Basic Med Sci, POB 84428, Riyadh 84428, Saudi Arabia

7.Univ Jeddah, Coll Med, Dept Anat, POB 8304, Jeddah 23234, Saudi Arabia

8.Jazan Univ, Coll Sci, Chem Dept, Female Sect, Jazan 82621, Saudi Arabia

关键词： Bovine; LATS 2 gene; Expression; Transcriptional regulation

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.554； 五年影响因子：2.564 ）

ISSN： 0034-5288

年卷期： 2022 年 152 卷

页码：

收录情况： SCI

摘要： As an important downstream effector gene in the hippo signaling pathway, large tumor suppressor gene 2 (LATS2) is involved in cell proliferation and differentiation, organ size and tissue regeneration, and plays an important role in regulating the growth and development of animal muscles. The purpose of this study is to explore the temporal expression of bovine LATS2 gene, and determine the key transcription factors for regulating bovine LATS2 gene. The result showed that bovine LATS2 gene was highly expressed in liver and longissimus dorsi, and was up-regulated in infancy muscle. In addition, it was highly expressed on the 2th day during the differentiation stage of myoblast. The upstream 1.7 Kb sequence of the 5 'translation region of bovine LATS2 gene was cloned, and 7 different deletion fragments were amplified by the upstream primers. These fragments were constructed into double luciferase reporter vectors and transfected into myoblasts and myotubes cells, respectively to detect the core promoter regions. In addition, the key transcription factors of the core promoter sequence of the bovine LATS2 gene were analyzed and predicted by online software. Combining with site -directed mutations, siRNA interference and chromatin immunoprecipitation technology, it was identified that MEF2A and MyoG combined in core promoter region (-248/-56) to regulate the transcription activity of bovine LATS2 gene. The results have laid a theoretical foundation for exploring the molecular regulation mechanism of LATS2 gene in the process of muscle growth.

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