Aptamer-Based Lateral Flow Test Strip for the Simultaneous Detection of Salmonella typhimurium, Escherichia coli O157:H7 and Staphylococcus aureus
文献类型： 外文期刊
作者： Lu, Chunxia 1 ; Gao, Xiaoxu 1 ; Chen, Ya 2 ; Ren, Jiangtao 2 ; Liu, Changbin 3 ;
作者机构： 1.Yangtze Normal Univ, Life Sci & Technol Inst, Chongqing, Peoples R China
2.Chongqing Fuling Inst Food & Drug Control, Chongqing 408100, Peoples R China
3.Xinjiang Acad Agr & Reclamat Sci, Key Labs Sheep Breeding & Reproduce, Wuyi Rd 221, Shihezi 832000, Peoples R China
关键词： Aptamer; Escherichia coli O157; H7; gold nanoparticles (AuNPs); lateral flow test strip; Salmonella typhimurium; Staphylococcus aureus
期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）
ISSN： 0003-2719
年卷期：
页码：
收录情况： SCI
摘要： An aptamer-based lateral flow test strip was developed for the simultaneous detection of Salmonella Typhimurium, Escherichia coli O157:H7, and Staphylococcus aureus. This assay was based on the sandwich-type format using primary aptamer conjugated with gold nanoparticles (AuNPs) as the signal probe and a secondary aptamer-coated membrane as a capture probe. Several experimental conditions that influenced sensitivity were investigated, including the aptamer and NaCl concentrations and the loaded amounts of gold nanoparticle-aptamer conjugates and streptavidin-aptamer conjugates. Under the optimal experimental conditions, the visual limits of detection of the strip for S. Typhimurium, E. coli O157:H7 and S. aureus were 10(3) CFU/mL, 10(4) CFU/mL, and 10(4) CFU/mL, respectively. All measurements were achieved within 10 min, and no significant cross-reactivity with several foodborne pathogens was observed. This aptamer-based strip was successfully applied to the simultaneous detection of these foodborne pathogens in food samples. The aptamer-based lateral flow test strip was shown to provide a reliable, simple and accurate tool for the simultaneous detection of multiple foodborne pathogens.
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