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Evaluation of a cuticle protein gene as a potential RNAi target in aphids

文献类型： 外文期刊

作者： Shang, Feng 1 ; Ding, Bi-Yue 1 ; Ye, Chao 1 ; Yang, Li 1 ; Chang, Teng-Yu 1 ; Xie, Jiaqin 3 ; Tang, Liang-De 4 ; Niu, Jin 1 ;

作者机构： 1.Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing, Peoples R China

2.Southwest Univ, Acad Agr Sci, Int Joint Lab China Belgium Sustainable Crop Pest, State Cultivat Base Crop Stress Biol Southern Mt, Chongqing, Peoples R China

3.Chongqing Univ, Sch Life Sci, Chongqing Engn Res Ctr Fungal Insecticide, Genet Engn Res Ctr, Chongqing, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China

关键词： cuticle protein; RNA interference; mortality; aphids; predator; biological control

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND RNA interference (RNAi) has potential as a pest insect control technique. One possible RNAi target is the cuticle protein, which is important in insect molting and development. As an example, here we evaluate the possibility of designing double-stranded RNA (RNA) that is effective for silencing the cuticle protein 19 gene (CP19) in aphids but is harmless to non-target predator insects. RESULTS The sequences of CP19s were similar (86.6-94.4%) among the tested aphid species (Aphis citricidus, Acyrthosiphon pisum, and Myzus persicae) but different in the predator Propylaea japonica. Ingestion of species-specific dsRNAs of CP19 by the three aphids produced 39.3-64.2% gene silencing and 45.8-55.8% mortality. Ingestion of non-species-specific dsRNA (dsAcCP19) by Ac. pisum and M. persicae gave gene silencing levels ranging from 40.4% to 50.3% and 43.3-50.8% mortality. The dsApCP19 did not affect PjCP19 expression or developmental duration in P. japonica. CONCLUSION The results demonstrate that CP19 is a promising RNAi target for aphid control via one dsRNA design. The targeting of genes that are conserved in insect pests but not present in beneficial insects is a useful RNAi-based pest control strategy. (c) 2019 Society of Chemical Industry

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[1]Evaluation of a cuticle protein gene as a potential RNAi target in aphids. Shang, Feng,Ding, Bi-Yue,Ye, Chao,Yang, Li,Chang, Teng-Yu,Niu, Jinzhi,Wang, Jin-Jun,Shang, Feng,Ding, Bi-Yue,Ye, Chao,Yang, Li,Chang, Teng-Yu,Niu, Jinzhi,Wang, Jin-Jun,Xie, Jiaqin,Tang, Liang-De. 2020

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