文献类型： 外文期刊
作者： Zhu, Bo 1 ; Han, Hongjuan 2 ; Fu, Xiaoyan 2 ; Li, Zhenjun 2 ; Gao, Jianjie 2 ; Yao, Quanhong 2 ;
作者机构： 1.Anhui Normal Univ, Coll Life Sci, Key Lab Conservat & Utilizat Important Biol Resou, Wuhu, Anhui, Peoples R China
2.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai, Peoples R China
关键词： nitroaromatic compound; biodegradation; contamination; phytotoxicity; fluorescence
期刊名称：PLANT SOIL AND ENVIRONMENT （ 影响因子：1.799； 五年影响因子：2.169 ）
ISSN： 1214-1178
年卷期： 2018 年 64 卷 8 期
页码：
收录情况： SCI
摘要： The explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. TNT is toxic to many organisms, it is known to be a potential human carcinogen, and is persistent in the environment. This study presents a system of phytoremediation by Arabidopsis plants developed on the basis of overexpression of NAD(P) H-flavin nitroreductase (NFSB) from the Sulfurimonas denitrificans DSM1251. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerance and a strikingly higher capacity to remove TNT from their media. The highest specific rate constant of TNT disappearance rate was 1.219 and 2.297 mL/g fresh weight/h for wild type and transgenic plants, respectively. Meanwhile, the nitroreductase activity in transgenic plant was higher than wild type plant. All this indicates that transgenic plants show significantly enhanced tolerances to TNT; transgenic plants also exhibit strikingly higher capabilities of removing TNT from their media and high efficiencies of transformation.
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