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A TLC-HPLC Method for Determination of Thiamphenicol in Pig, Chicken, and Fish Feedstuffs

文献类型： 外文期刊

作者： Wang, JianMei 1 ; Yang, JinJing 2 ; Qian, MingRong 1 ; Zhou, DanNa 3 ; Ke, XianBing 2 ; Sun, GuiZhi 2 ; Yang, Bo 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Inst Qual & Stand Agroprod, Hangzhou 310021, Zhejiang, Peoples R China

2.Wuhan Inst Bioengn, Hubei Engn Res Ctr Viral Vector, 1 Hanshi Rd, Wuhan 430415, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr, Anim Husb & Vet Inst, Wuhan 430064, Hubei, Peoples R China

关键词： High-performance liquid chromatography; Thin-layer chromatography; Thiamphenicol; Animal feedstuffs

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN： 1936-9751

年卷期： 2018 年 11 卷 11 期

页码：

收录情况： SCI

摘要： An effective thin-layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of thiamphenicol (TAP) in pig, chicken, and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C-18 column using an isocratic procedure with acetonitrile-water (21.7:78.3, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The TAP concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=162,630x-2381.7, r>0.9998) were achieved within the concentration range of 0.05-10.00g/mL. The recoveries of TAP spiked at levels of 1, 10, and 100g/g ranged from 82.0 to 114.9% with the intra-day and inter-day relative standard deviation (RSD) less than 9.0%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.03mg/kg for pig feedstuffs, 0.02 and 0.04mg/kg for chicken feedstuffs, and 0.02 and 0.03mg/kg for fish feedstuffs, respectively. This reliable, simple, and cost-effective method could be applied to the routine monitoring of TAP in animal feedstuffs.

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