Tissue Expression Analysis, Cloning, and Characterization of the 5 '-Regulatory Region of the Bovine LATS1 Gene
文献类型： 外文期刊
作者： Wei, Dawei 1 ; Raza, Sayed Haidar Abbas 3 ; Wang, Xingping 1 ; Khan, Rajwali 4 ; Lei, Zhaoxiong 1 ; Zhang, Guijie 1 ; Zhang, Jiupan 5 ; Luoreng, Zhuoma 1 ; Ma, Yun 1 ; Alamoudi, Muna O. 6 ; Aloufi, Bandar Hamad 6 ; Alshammari, Ahmed Mohajja 6 ; El-Aziz, Ayman Hassan Abd 7 ; Alhomrani, Majid 8 ; Alamri, Abdulhakeem S. 8 ;
作者机构： 1.Ningxia Univ, Sch Agr, Yinchuan, Peoples R China
2.Key Lab Ruminant Mol Cell Breeding, Yinchuan, Ningxia Hui, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Peoples R China
4.Univ Agr, Dept Livestock Management Breeding & Genet, Peshawar, Pakistan
5.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan, Peoples R China
6.Univ Hail, Coll Sci, Dept Biol, Hail, Saudi Arabia
7.Damanhour Univ, Fac Vet Med, Anim Husb & Anim Wealth Dev Dept, Damanhour, Egypt
8.Taif Univ, Fac Appl Med Sci, Dept Clin Labs Sci, Taif, Saudi Arabia
9.Taif Univ, Ctr Biomed Sci Res CBSR, Deanship Sci Res, Taif, Saudi Arabia
关键词： LATS1 gene; expression; core promoter; transcription; factor
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）
ISSN：
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： As a member of the large tumor suppressor (LATS) gene family, LATS1 plays an important role in regulating muscle growth and development. In this study, we determined the distinct exhibit patterns of tissue expression of bovine LATS1. Further, we determined the functional proximal minimal promoter of bovine LATS1 and identified the key transcription factors in the core promoter region to elucidate its molecular regulation mechanism. The results showed that bovine LATS1 was highly expressed in the longissimus thoracis and upregulation in infancy muscle. An electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay in combination with site-directed mutation and small interfering RNA (siRNA) interference demonstrated that myogenic differentiation 1 (Myod1) and myocyte enhancer factor 2A (MEF2A) binding in the core promoter region (-298/-123 bp) play important roles in the transcriptional regulation of the bovine LATS1 promoter. Taken together, these interactions provide insight into the regulatory mechanisms of LATS1 transcription in mediating skeletal muscle growth in cattle.
- 相关文献
作者其他论文 更多>>
-
Regulatory role of exosome-derived miRNAs and other contents in adipogenesis
作者：Song, Xiaoyu;Song, Yaping;Hu, Yamei;Zhang, Lingkai;Jiang, Chao;Ma, Yanfen;Ma, Yun;Wu, Hao;Wei, Dawei;Song, Xiaoyu;Song, Yaping;Hu, Yamei;Zhang, Lingkai;Jiang, Chao;Ma, Yanfen;Ma, Yun;Wu, Hao;Wei, Dawei;Zhang, Jiupan;Huang, Zengwen;Raza, Sayed Haidar Abbas;Raza, Sayed Haidar Abbas
关键词：Exosome; miRNAs; Adipogenesis; IMF; ncRNAs; Adipose; Muscle
-
FOXO1 regulates bovine skeletal muscle cells differentiation by targeting MYH3
作者：Jiang, Chao;Song, Yaping;Song, Xiaoyu;Wu, Hao;Jiao, Ruopu;Li, Lanlan;Zhang, Guijie;Wei, Dawei;Jiang, Chao;Song, Yaping;Song, Xiaoyu;Wu, Hao;Jiao, Ruopu;Li, Lanlan;Zhang, Guijie;Wei, Dawei;Zhang, Jiupan
关键词：Bovine; FOXO1; Myogenesis; RNA-seq; MYH3
-
The Effect of Replacing Whole-Plant Corn Silage with Daylily on the Growth Performance, Slaughtering Performance, Muscle Amino Acid Composition, and Blood Composition of Tan Sheep
作者：Zhang, Junli;Yang, Yuwei;Wang, Xiuqin;Li, Fen;Na, Rina;Bai, Xue;Ma, Yanfen;Ma, Yun
关键词：daylily silage; tan sheep; growth performance; amino acids; blood biochemical indicators
-
FOXO1 regulates the formation of bovine fat by targeting CD36 and STEAP4
作者：Song, Yaping;Jiang, Chao;Song, Xiaoyu;Wu, Hao;Zhang, Juan;Zhang, Lingkai;Cai, Bei;Wang, Xingping;Reng, Zhuoma Luo;Ma, Yun;Wei, Dawei;Song, Yaping;Jiang, Chao;Song, Xiaoyu;Wu, Hao;Zhang, Juan;Zhang, Lingkai;Cai, Bei;Wang, Xingping;Reng, Zhuoma Luo;Ma, Yun;Wei, Dawei;Zhang, Jiupan;Raza, Sayed Haidar Abbas;Zhang, Le
关键词：Bovine; FOXO1; Adipocytes; RNA-seq; Metabolome
-
Interaction of C/EBP beta with SMAD2 and SMAD4 genes induces the formation of lipid droplets in bovine myoblasts
作者：Wei, Dawei;Zhang, Guijie;Zhang, Le;Raza, Sayed Haidar Abbas;Raza, Sayed Haidar Abbas;Zhang, Jiupan;Liang, Xiaojun;Juan, Zhao;Al-Amrah, Hadba;Al Abdulmonem, Waleed;Alharbi, Yousef Mesfer
关键词：SMAD2; SMAD4; C/EBP beta; Transcriptional regulation; Myoblast
-
Influence of Parturition on Rumen Bacteria and SCFAs in Holstein Cows Based on 16S rRNA Sequencing and Targeted Metabolomics
作者：Guo, Yansheng;Wang, Feifei;Mao, Yongxia;Kong, Weiyi;Zhang, Guijie;Wang, Jiandong
关键词：parturition; dairy cows; rumen bacterial communities; short-chain fatty acids
-
Roles of MEF2A and MyoG in the transcriptional regulation of bovine LATS2 gene
作者：Zhang, Jiupan;Jin, Wang;Wei, Dawei;Yaping, Song;Chao, Jiang;Raza, Sayed Haidar Abbas;Almohaimeed, Hailah M.;Batarfi, Munirah A.;Assiri, Rasha;Aggad, Waheeb S.;Ghalib, Samirah H.;Ageeli, Abeer A.
关键词：Bovine; LATS 2 gene; Expression; Transcriptional regulation
