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CpxAR of Actinobacillus pleuropneumoniae Contributes to Heat Stress Response by Repressing Expression of Type IV Pilus Gene apfA

文献类型： 外文期刊

作者： Liu, Feng 1 ; Peng, Wei 1 ; Yan, Kang 1 ; Huang, Jing 5 ; Yuan, Fangyan 4 ; He, Qigai 1 ; Chen, Huanchun 1 ; Bei, Weicheng 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China

2.Yangtze Univ, Coll Anim Sci, Jingzhou, Hubei, Peoples R China

3.Hubei Hongshan Lab, Wuhan, Peoples R China

4.Hubei Acad Agr Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr, Inst Anim Husb & Vet Sci, Wuhan, Peoples R China

5.Zhejiang Gongshang Univ, Sch Foreign Languages, Hangzhou, Zhejiang, Peoples R China

关键词： Actinobacillus pleuropneumoniae; heat stress; TCS; CpxAR; type IV pilus

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2022 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Acute pleuropneumonia in swine, caused by Actinobacillus pleuropneumoniae, is characterized by a high and sustained fever. Fever creates an adverse environment for many bacteria, leading to reduced bacterial proliferation; however, most pathogenic bacteria can tolerate higher temperatures. CpxAR is a two-component regulation system, ubiquitous among Gram-negative bacteria, which senses and responds to envelope alterations that are mostly associated with protein misfolding in the periplasm. Our previous study showed that CpxAR is necessary for the optimal growth of Actinobacillus pleuropneumoniae under heat stress. Here, we showed that mutation of the type IV pilin gene apfA rescued the growth defect of the cpxAR deletion strain under heat stress. RNA sequencing (RNAseq) analyses revealed that 265 genes were differentially expressed in the DcpxAR strains grown at 42 degrees C, including genes involved in type IV pilus biosynthesis. We also demonstrated direct binding of the CpxR protein to the promoter of the apf operon by an electrophoretic mobility shift assay and identified the binding site by a DNase I footprinting assay. In conclusion, our results revealed the important role of CpxAR in A. pleuropneumoniae resistance to heat stress by directly suppressing the expression of ApfA. IMPORTANCE Heat acts as a danger signal for pathogens, especially those infecting mammalian hosts in whom fever indicates infection. However, some bacteria have evolved exquisite mechanisms to survive under heat stress. Studying the mechanism of resistance to heat stress is crucial to understanding the pathogenesis of A. pleuropneumoniae during the acute stage of infection. Our study revealed that CpxAR plays an important role in A. pleuropneumoniae resistance to heat stress by directly suppressing expression of the type IV pilin protein ApfA.

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