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Isolation and functional characterization of the ShCBF1 gene encoding a CRT/DRE-binding factor from the wild tomato species Solanum habrochaites

文献类型： 外文期刊

作者： Li, Zhenjun 1 ; Zhang, Lili 2 ; Li, Jingfu 1 ; Xu, Xiangyang 1 ; Yao, Quanhong 4 ; Wang, Aoxue 1 ;

作者机构： 1.Northeast Agr Univ, Coll Hort, Harbin 150030, Peoples R China

2.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

3.Northeast Agr Univ, Heilongjiang Prov Key Univ Lab Agr Biol Funct Gen, Harbin 150030, Peoples R China

4.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

关键词： CRT/DRE;High salinity;Low temperature;ShCBF1;Solanum habrochaites

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

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收录情况： SCI

摘要： Plant growth and productivity are greatly affected by low ambient temperature. Complex cascades of gene expression in cold stress response are regulated by transcription factors. In this study, a cDNA clone, named ShCBF1, was isolated from Solanum habrochaites seedlings (a wild relative of cultivated tomato). It was classified as one of CBF family members based on multiple sequence alignment. The expression analysis confirmed that ShCBF1 was induced by low temperature, high salinity and drought stress. Experiments of subcellular localization in tobacco leaf cells indicated that it was localized in nucleus. Transient expression assay using onion epidermal cells revealed that the ShCBF1 protein could function similarly to AtCBF1 in activating the expression of reporter genes with a CRT/DRE element in their promoter. Moreover, ectopic overexpression of ShCBF1 in Arabidopsis enhanced freezing and high salinity tolerance of transgenic plants by improving the expression levels of some stress-responsive marker genes. Taken together, our results suggest that ShCBF1 behaves as a typical plant CBF transcription factor and might be involved in plant response to various environmental stresses.

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[1]Exogenous 6-benzylaminopurine confers tolerance to low temperature by amelioration of oxidative damage in eggplant (Solanum melongena L.) seedlings. Chen, Jianlin,Wu, Xuexia,Zhu, Zongwen,Xu, Shuang,Zha, Dingshi,Wu, Xuexia,Zhu, Zongwen,Xu, Shuang,Zha, Dingshi,Yao, Xinfeng. 2016

[2]Protection of photosynthesis and antioxidative system by 24-epibrassinolide in Solanum melongena under cold stress. Wu, X. X.,Zha, D. S.,Wu, X. X.,Zhu, Z. W.,Yang, S. J.,Zha, D. S.,He, J.. 2014

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