Engineering higher yield and herbicide resistance in rice by Agrobacterium-mediated multiple gene transformation
文献类型： 外文期刊
作者： Cao, MX 1 ; Huang, JQ 2 ; Wei, ZM 2 ; Yao, QH 2 ; Wan, CZ 2 ; Lu, JA 2 ;
作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China
期刊名称：CROP SCIENCE （ 影响因子：2.319； 五年影响因子：2.631 ）
ISSN： 0011-183X
年卷期： 2004 年 44 卷 6 期
页码：
收录情况： SCI
摘要： The Vitreoscilla hemoglobin gene (VHb), trans-zeatin secretion gene (tzs), and the modified 5-enolpyruvyishikimate-3-phosphate synthase gene (EPSPS), as linked expression cassettes, were simultaneously introduced into immature embryos of the rice (Oryza sativa L.) cultivars Xiushui-11, Qiufeng, Youfeng, and Hanfeng by Agrobacterium tumefaciens. A total of 1153 transgenic lines composed of 4222 plants were obtained through selection for hygromycin (hyg) B resistance. Genomic polymerase chain reaction (PCR), southern and northern blotting analyses, and other relative tests showed that all transgenes had been integrated into the rice genome and expressed effectively. Approximately 90.2% of the transgenic lines harbored all the transgenes. Expression analysis revealed that all transgenes coexpressed stably in transgenic plants, and the frequency of coexpression was about 85%. Statistically significant increases were observed in plant height, particle length, total grains per panicle, and filled grains per panicle in transgenic plant lines compared with the control. Our study demonstrates a possible way to introduce different transgenes as linked expression cassettes within a single vector into the plant genome. Moreover, this transgenic approach has great potential in developing new rice cultivars with increased productivity and enhanced tolerance to the herbicide glyphosate.
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