Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development
文献类型: 外文期刊
作者: Ye, R 1 ; Yao, QH 2 ; Xu, ZH 2 ; Xue, HW 2 ;
作者机构: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant ol Genet, Shanghai 200032, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant ol Genet, Shanghai 200032, Peoples R China; Max Planck Inst Mol Plant Physiol Plant Mol Physi, Partner Grp, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China
关键词: transcription factors;activation;Oryza sativa;seed development
期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )
ISSN: 0960-7412
年卷期: 2004 年 38 卷 2 期
页码:
收录情况: SCI
摘要: An efficient yeast-based system was developed for the isolation of plant cDNAs encoding transcription factors (TFs) and proteins with transcription activation functions (co-activators). The system consists of two vectors: (i) a reporter vector (pG221) harboring the iso-1-cytochrome c (CYC1) core promoter and the beta-galactosidase (lacZ) gene; and (ii) a cDNA library construction vector (pYF503), which yields a library of plant peptides fused to the GAL4-binding domain (GAL4-BD). Expression of a peptide harboring the characteristics of a transcriptional activator leads to expression of lacZ, allowing for selection of relevant colonies. TFs during rice embryo development were isolated through this system. Approximately 200 confirmed positive colonies were obtained from screening 10(6) yeast colonies, and sequence analysis of conserved domains identified 75 independent cDNAs, 20 of which encoded plant TFs or co-activators, including members of the APETALA2 (AP2)/ethylene-responsive element-binding protein (EREBP), MYB and growth-regulating factor (GRF) families. Peptides encoded by 13 of the isolated cDNAs were classified as potential TFs or co-activators because of the presence of conserved TF-like domains. Additionally, 2, 11, and 13 clones encoded kinases, chromosome-related proteins, and unknown proteins, respectively, while the remaining 16 cDNAs were associated with specific functions seemingly unrelated to TFs. Expression pattern analysis of selected TF-encoding genes via RT-PCR revealed that these genes were expressed during seed development, with differential transcription observed during various stages. This work provides informative hints for further study of the regulatory mechanism of rice seed development and illustrates an identification strategy that will be of practical value for the isolation of TFs and co-activators associated with specific plant developmental processes.
- 相关文献
作者其他论文 更多>>
-
Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase
作者:Cao, MX;Huang, JQ;Yao, QH;Liu, SJ;Wang, CL;Wei, ZM
关键词:NITS;cell-permeable Cre recombinase;marker-free;transgenic rice plants;biosafety
-
Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola
作者:Peng, RH;Yao, QH;Xiong, AS;Cheng, ZM;Li, Y
关键词:Agrobacterium-mediated transformation;Aspergillus phytase;Brassica napus;codon modification;endoplasmic reticulum retention signal
-
Agrobacterium-mediated multiple gene transformation in rice using a single vector
作者:Cao, MX;Huang, JQ;Wei, ZM;Yao, QH;Wan, CZ;Lu, JA
关键词:Agrobacterium tumefaciens;linked expression cassettes;multiple genes;rice;transformation
-
Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato
作者:Xiong, AS;Yao, QH;Peng, RH;Li, X;Han, PL;Fan, HQ
关键词:RNA interference;ACC oxidase;ethylene;shelf life;tomato
-
Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage
作者:Li, X;Peng, RH;Fan, HQ;Xiong, AS;Yao, QH;Cheng, ZM;Li, Y
关键词:Agrobacterium tumefaciens;cabbage;flooding tolerance;plant hemoglobin;vhb gene
-
Isolation and characterization of a cDNA encoding two novel heat-shock factor OsHSF6 and OsHSF12 in Oryza sativa L.
作者:Liu, JG;Yao, QH;Zhang, Z;Peng, RH;Xiong, AS;Xu, F;Zhu, H
关键词:heat-shock factor;OsHSF6;OsHSF12;rice;yeast one-hybrid
-
A new rice zinc-finger protein binds to the O2S box of the alpha-amylase gene promoter
作者:Peng, RH;Yao, QH;Xiong, AS;Fan, HQ;Li, X;Peng, YL;Cheng, ZM;Li, Y
关键词:rice zinc-induced protein;O2S box;yeast one-hybrid system