Ubiquitin-conjugating enzyme (E-2) confers rice UV protection through phenylalanine ammonia-lyase gene promoter unit
文献类型： 外文期刊
作者： Peng, RH 1 ; Yao, QH 2 ; Xiong, AS 2 ; Fan, HQ 2 ; Li, X 2 ; Peng, YL 2 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, AgroBiotechnol Res Ctr, Shanghai 201106, Peoples R China
2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, AgroBiotechnol Res Ctr, Shanghai 201106, Peoples R China; China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China
关键词： rice ubiquitin-conjugating enzyme;UV protection;cis-element;yeast one-hybrid system
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN： 0577-7496
年卷期： 2003 年 45 卷 11 期
页码：
收录情况： SCI
摘要： The induction of general phenylpropanoid and flavonoid glycoside pathways and the consequential accumulation of UV protective flavonoids so far have been regarded as the only major metabolic response to UV light. Phenylalanine ammonia-lyase (PAL) is a key enzyme of phenylpropanoid metabolism. Inducible in vivo footprints in the pal - 1 promoter defined the motif CTCCAACAACCCCTTC as a cis-element that transmits the UV signal. In order to clone its corresponding trans-acting element, three copies of the motif were synthesized and used as a bait of yeast one-hybrid system. A number of genes coding ubiquitin-conjugating enzyme-like proteins (E-2) were isolated from a rice cDNA library by the system. The rice (Oryza sativa L.) E-2 (RE2) was high homology to other E-2 of different species and can be induced by UV light. RE2 can react with nuclear extraction protein and binds the pal - I promoter cis-element. These results suggest that RE2 takes part in plant response to UV stress through regulating the phenylpropanoid metabolism.
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