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Quantum Dot Submicrobead-Based Immunochromatographic Assay for the Determination of Parathion in Agricultural Products

文献类型： 外文期刊

作者： Liu, Beibei 1 ; Li, Pan 1 ; Wang, Yulong 1 ; Guo, Yirong 3 ; Zhang, Hanxiaoya 1 ; Dong, Sa 1 ; Xiong, Yonghua 5 ; Zhang, 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Safety State Key Lab Cultivat Base, Minist Sci & Technol,Minist Agr,Inst Food Safety, Key Lab Control Technol & Stand Agroproduct Safet, Nanjing 210014, Peoples R China

3.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Peoples R China

4.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

5.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China

关键词： Quantum dot submicrobead; Immunochromatographic assay; Parathion; Quantitative detection

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN： 1936-9751

年卷期： 2020 年 13 卷 9 期

页码：

收录情况： SCI

摘要： Due to high toxicity to mammals and hazard to the ecosystem, parathion has been banned or limited for use in many countries. It is essential to ensure food safety by parathion suspecting. On-site testing, as a critical component of the food chain safe system, plays a major role in the protection of food safety and human health. In this study, based on monoclonal antibody (Mab), a rapid and sensitive quantum dot submicrobead-based immunochromatographic assay (QB-ICA) was developed for the on-site determination of parathion in agricultural products. The established QB-ICA exhibited good linearity over the range of parathion from 0.7 to 13.0 ng mL(-1), with half inhibitory concentration (IC50) of 2.48 ng mL(-1)and visual detection limits of 6.25 ng mL(-1). The testing process could be accomplished within 15 min, and no cross-reactivities with other organophosphorus pesticides were observed. Three kinds of agricultural products (rice, vegetable, and apple) were spiked and analyzed via QB-ICA for method validation; meanwhile, the comparison was conducted between QB-ICA and gas chromatography (GC). The results revealed that the recovery ranged from 85.23 to 111.4%, with the coefficient of variations (CV) less than 11%, suggesting that the developed QB-ICA is practical and reliable for use. This study provides an alternative approach for on-site detection of parathion, which shows great promise to be further used in the surveillance of food and the environment.

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