Development of a real-time recombinase polymerase amplification assay for rapid detection of Aeromonas hydrophila
文献类型： 外文期刊
作者： Qu, Yang 1 ; Wang, Qing 1 ; Li, Yingying 1 ; Wang, Yingying 1 ; Yin, Jiyuan 1 ; Ren, Yan 1 ; Liu, Chun 3 ; Liu, Xiaofang; 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol Guangdong Prov, Minist Agr,Pearl River Fisheries Res Inst, Guangzhou, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
3.Zhongkai Univ Agr & Engn, Innovat Inst Anim Hlth Breeding, Coll Anim Sci & Technol, Guangzhou, Peoples R China
4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China
关键词： Aeromonas hydrophila; detection; haemolysin gene; real‐ time recombinase polymerase amplification
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期：
页码：
收录情况： SCI
摘要： Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real-time recombinase polymerase amplification (real-time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37 degrees C for 20 min and was highly specific with no cross-reaction with other fish pathogens or with other Aeromonas species. The assay detection limit was 10(2) copies of the Aeromonas hydrophila per reaction. Compared with traditional culture-based method or real-time PCR, the diagnostic sensitivity and specificity of the real-time RPA were 73.7 and 100%, as well as 64.7 and 93%. Our newly developed real-time RPA was specific and sensitive and can be used in large-scale and point-of-care field investigations of A. hydrophila infections to enable earlier diagnoses.
- 相关文献
作者其他论文 更多>>
-
Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection
作者：Wu, Huiliang;Yin, Jiyuan;Jiang, Peng;Wang, Linchuan;Wang, Qing;Li, Siming;Yan, Han;Wang, Hao;Ding, Zhaoyang;Li, Pengfei;Chen, Bo;Wang, Qing;Wang, Qing
关键词：Grass carp reovirus; VP4; Oral immunization; L. lactis; Immunogenicity; Protective efficacy
-
Establishment and characterization of a kidney cell line from hybrid snakehead (male Channa argus x female Channa maculata ) and its susceptibility to hybrid snakehead rhabdovirus (HSHRV)
作者：Deng, Huiling;Zheng, Shucheng;Li, Yingying;Mo, Xubing;Zhao, Jian;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Wang, Yingying;Deng, Huiling;Zheng, Shucheng;Wang, Qing;Wang, Yingying
关键词：CAMK cell line; Hybrid snakehead; Virology; Hybrid snakehead rhabdovirus
-
A comprehensive study of the colloidal properties, biocompatibility, and synergistic antioxidant actions of Antarctic krill phospholipids
作者：Su, Dong;Zhang, Zipeng;Zhang, Yating;Zhao, Ling;Yu, Yueqin;Su, Dong;Wang, Xixi;Liu, Xiaofang;Miao, Junkui;Yu, Yuan;Leng, Kailiang;Leng, Kailiang;Leng, Kailiang
关键词：Antarctic krill; PUFAs-esterified phospholipid; Antioxidant; Synergy; Oxygen-sensitive
-
Comparative metabolomics study on the quality of Antarctic krill (Euphausia superba) stored at different temperatures
作者：Ma, Xinyu;Su, Dong;Xie, Wancui;Ma, Xinyu;Su, Dong;Zhu, Jiancheng;Miao, Junkui;Liu, Xiaofang;Leng, Kailiang;Wang, Xixi;Leng, Kailiang
关键词：Antarctic krill; metabolomics; quality biomarkers; short-term storage; temperature variation
-
Interferon regulatory factors inhibit TiLV replication by activating interferon-a3 in tilapia (Oreochromis niloticus)
作者：Ke, Zishan;Wen, Jing;Wang, Yingying;Li, Bo;Zhang, Defeng;Mo, Xubing;Li, Yingying;Ren, Yan;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Zheng, Shucheng;Ke, Zishan;Li, Bo;Zheng, Shucheng;Wu, Siyu;Zheng, Shucheng
关键词：Interferon regulatory factor; Interferon; Innate immunity; Tilapia; Tilapia lake virus
-
Development of real-time recombinase polymerase amplification (RPA) and RPA combined with lateral flow dipstick (LFD) assays for the rapid and sensitive detection of cyprinid herpesvirus 3
作者：Li, Yingying;Li, Ruifan;Mo, Xubing;Wang, Yingying;Yin, Jiyuan;Ren, Yan;Pan, Houjun;Shi, Cunbin;Zhang, Defeng;Wang, Qing;Bergmann, Sven M.;Zhang, Defeng;Wang, Qing
关键词：cyprinid herpesvirus 3(CyHV-3); real-time RPA; RPA-LFD; sera
-
Unlocking Eco-solutions: Analyzing parasitic disease resistance in Yellow River carp (Cyprinus carpio haematopterus) under different ecological treatments
作者：Pan, Houjun;Li, Yingying;Liang, Hongru;Wang, Yajun;Xie, Jun;Shi, Cunbin;Lv, Jun;Zhang, Yuanyuan;Wang, Xianke;Li, Xudong;Pan, Houjun;Shi, Cunbin
关键词：Fish; Intensive aquaculture; Mixed culture; Parasitosis occurrence; Logistic regression analysis
