Development of a real-time recombinase polymerase amplification assay for rapid detection of Aeromonas hydrophila
文献类型： 外文期刊
作者： Qu, Yang 1 ; Wang, Qing 1 ; Li, Yingying 1 ; Wang, Yingying 1 ; Yin, Jiyuan 1 ; Ren, Yan 1 ; Liu, Chun 3 ; Liu, Xiaofang; 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol Guangdong Prov, Minist Agr,Pearl River Fisheries Res Inst, Guangzhou, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
3.Zhongkai Univ Agr & Engn, Innovat Inst Anim Hlth Breeding, Coll Anim Sci & Technol, Guangzhou, Peoples R China
4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan, Peoples R China
关键词： Aeromonas hydrophila; detection; haemolysin gene; real‐ time recombinase polymerase amplification
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期：
页码：
收录情况： SCI
摘要： Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real-time recombinase polymerase amplification (real-time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37 degrees C for 20 min and was highly specific with no cross-reaction with other fish pathogens or with other Aeromonas species. The assay detection limit was 10(2) copies of the Aeromonas hydrophila per reaction. Compared with traditional culture-based method or real-time PCR, the diagnostic sensitivity and specificity of the real-time RPA were 73.7 and 100%, as well as 64.7 and 93%. Our newly developed real-time RPA was specific and sensitive and can be used in large-scale and point-of-care field investigations of A. hydrophila infections to enable earlier diagnoses.
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