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Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-kappa B/bFGF signaling

文献类型： 外文期刊

作者： Guo, Jun-Li 2 ; Zheng, Shao-Jiang 2 ; Li, Yue-Nan 2 ; Jie, Wei; Hao, Xin-Bao 2 ; Li, Tian-Fa 2 ; Xia, Li-Ping 2 ; Mei, 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Haikou 571101, Hainan Province, Peoples R China

2.Hainan Med Coll, Affiliated Hosp, Canc Res Inst, Key Lab Trop Med, Haikou 571199, Hainan Province, Peoples R China

3.Hainan Med Coll, Affiliated Ho

关键词： Anti-migration;Anti-proliferation;Basic fibroblast growth factor;Gastric cancer;Nuclear factor-kappa B;Toxicarioside A

期刊名称：WORLD JOURNAL OF GASTROENTEROLOGY （ 影响因子：5.742； 五年影响因子：5.044 ）

ISSN： 1007-9327

年卷期： 2012 年 18 卷 14 期

页码：

收录情况： SCI

摘要： AIM: To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line (SGC-7901) and determine the underlying molecular mechanism. METHODS: After SGC-7901 cells were treated with toxicarioside A at various concentrations (0.5, 1.5, 4.5, 9.0 mu g/mL) for 24 h or 48 h, cell viability was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the motility and invasion of tumor cells were assessed by the Transwell chamber assay. Immunofluorescence staining, reverse transcription polymerase chain reaction and Western blotting were performed to detect the expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR1), and nuclear factor-kappa B (NF-kappa B) activation was examined by electrophoretic mobility shift assay. RESULTS: The results showed that toxicarioside A was capable of reducing cell viability, inhibiting cell growth, and suppressing cell migration and invasion activities in a time- and dose-dependent manner in SGC-7901 cells. Further analysis revealed that not only the expression of bFGF and its high-affinity receptor FGFR1 but also the NF-kappa B-DNA binding activity were effectively blocked by toxicarioside A in a dose-dependent manner compared with the control group (P < 0.05 or P < 0.01). Interestingly, application of the NF-kappa B specific inhibitor, pyrrolidinedithiocarbamate (PDTC), to SGC-7901 cells significantly potentized the toxicarioside A-induced down-regulation of bFGF compared with the control group (P < 0.05). CONCLUSION: These findings suggest that toxicarioside A has an anti-gastric cancer activity and this effect may be achieved partly through down-regulation of NF-kappa B and bFGF/FGFR1 signaling. (C) 2012 Baishideng. All rights reserved.

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[1]Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-beta signaling in a bone marrow stromal cell line. Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Guo, Jun-Li,Tan, Guang-Hong. 2012

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