您好，欢迎访问宁夏农林科学院 机构知识库！
宁夏农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Interaction of MyoD and MyoG with Myoz2 gene in bovine myoblast differentiation

文献类型： 外文期刊

作者： Wei, Dawei 1 ; Zhang, Jiupan 2 ; Raza, Sayed Haidar Abbas 3 ; Song, Yaping 1 ; Jiang, Chao 1 ; Song, Xiaoyu 1 ; Wu, Hao 1 ; Alotaibi, Mashael Alhumaidi 4 ; Albiheyri, Raed 5 ; Al-Zahrani, Majid 6 ; Makhlof, Raafat T. M. 7 ; Alsaad, Mohammad A. 7 ; Abdelnour, Sameh A. 10 ; Quan, Guobo 11 ;

作者机构： 1.Ningxia Univ, Sch Agr, 489 Helanshan West Rd, Yinchuan 750021, Ningxia, Peoples R China

2.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750021, Ningxia, Peoples R China

3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China

4.Jouf Univ, Coll Sci, Biol Dept, POB 2014, Sakaka, Saudi Arabia

5.King Abdulaziz Univ, Dept Biol Sci, Fac Sci, Jeddah, Saudi Arabia

6.King Abdulaziz Univ, Coll Sci & Art, Biol Sci Dept, Rabigh, Saudi Arabia

7.Umm Al Qura Univ, Fac Med, Dept Parasitol, POB 715, Mecca 21955, Saudi Arabia

8.Minia Univ, Fac Med, Dept Parasitol, Al Minya, Egypt

9.King Abdulaziz Univ, Ctr Excellence Bionanosci Res, Jeddah, Saudi Arabia

10.Zagazig Univ, Fac Agr, Dept Anim Prod, Zagazig 44511, Egypt

11.Yunnan Anim Sci & Vet Inst, Kunming, Yunnan, Peoples R China

关键词： Promoter; MyoD; MyoG; Myoz2 gene

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.554； 五年影响因子：2.564 ）

ISSN： 0034-5288

年卷期： 2022 年 152 卷

页码：

收录情况： SCI

摘要： This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (-159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region-159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：宁夏农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台