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An aggregation-induced emission immunoassay for broad detection of polychlorinated biphenyls in chicken and crab

文献类型： 外文期刊

作者： Han, Chang 1 ; Wang, Yulong 2 ; Liu, Pengyan 2 ; Li, Pan 2 ; Liu, Beibei 2 ; Ding, Ning 3 ; Routledge, Michael N. 1 ; Liu, Zhengjiang 6 ; Zhang, Cunzheng 1 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety State, Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China

3.Inspect & Testing Ctr, Inst Agr Prod Qual, Suqian 223801, Peoples R China

4.Univ Leeds, Sch Med, Leeds LS2 9JT, W Yorkshire, England

5.Jiangsu Univ, Jiangsu Educ Dept, Int Joint Res Lab Intelligent Agr & Agri Prod Pro, Zhenjiang 212013, Jiangsu, Peoples R China

6.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China

关键词： Polychlorinated biphenyls; Broad-recognition monoclonal antibody; Immunoassay based on aggregation-induced emission nanobeads

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 2021影响因子：4.478； 五年影响因子：4.29 ）

ISSN： 1618-2642

年卷期： 2022 年 414 卷 17 期

页码：

收录情况： SCI

摘要： Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPS) with multiple variants, which may be harmful to human health by absorption and bioaccumulation. To ensure food safety, it is necessary to develop multi-residue immunoassays for broad recognition of PCBs. In this study, by mimicking the generic core structure of PCBs, three haptens have been designed and synthesized for monoclonal antibody (mAb) generation. A carboxylic acid derivative of PCB80 was a hapten that induced a mAb with broad recognition of PCBs. The results of ELISA further identified that the mAb could recognize 11 different kinds of PCBs; half-maximal inhibition concentrations (IC50) ranged from 33.12 to 476.42 ng/mL. Subsequently, using aggregation-induced emission luminogen (AlEgen) nanobeads as the tracer for the output signal, the IC50 value of the various PCBs was improved to 6.38-252.1 ng/mL. The limit of detection (LOD) varied from 0.32 to 42.15 ng/mL. Recoveries of 76.90-95.74% and intra-assay coefficients of variation of 8.5-14.4% were obtained with spiked chicken and crab meat samples. Matrix interference was eliminated by dilution, and no false-positive and false-negative results were observed. The developed assay provides a simple, broad-spectrum, and sensitive tool for detecting PCBs, with high-throughput possibilities for large-scale screening of PCBs in food.

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